Ugrás a tartalomra
Merck
Összes fotó(1)

Fontos dokumentumok

H8629

Sigma-Aldrich

Hexokinase and Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)

lyophilized powder

Szinonimák:

HK/G6PD

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez

Méret kiválasztása

500 UNITS
49 500,00 Ft
5000 UNITS
398 000,00 Ft

49 500,00 Ft

Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.
Megrendelés igénylése nagy tételben
Összes fotó(1)

Méret kiválasztása

Nézet módosítása
500 UNITS
49 500,00 Ft
5000 UNITS
398 000,00 Ft

About This Item

MDL-szám:
MFCD00131322
UNSPSC kód:
12352204
eCl@ss:
32160410
NACRES:
NA.54

49 500,00 Ft

Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.
Megrendelés igénylése nagy tételben

Forma

lyophilized powder

Minőségi szint

200

glucose-6-phosphate dehydrogenase activity

≥75 units/mg protein (biuret)

hexokinase activity

≥65 units/mg protein

tisztítva

crystallization

tárolási hőmérséklet

−20°C

Általános leírás

Research area: Cell signaling

Hexokinase and Glucose-6-phosphate Dehydrogenase are intracellular enzymes found distributed widely in the living cells.[1]
Enzyme Commission numbers 2.7.1.1 + 1.1.1.49

Alkalmazás

Hexokinase and Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae) has been used to evaluate the adenylate kinase (Adk), activities of the mutant Adk’s.[1] It has also been used for the determination of glucose and ATP.[2]

Biokémiai/fiziológiai hatások

Hexokinase initiates the glycolysis pathway by catalyzing the phosphorylation of glucose to Glucose-6-phosphate in the presence of ATP. Glucose-6-phosphate dehydrogenase plays a role in the subsequent oxidation of Glucose-6-phosphate in the presence of triphosphopyridine nucleotide (TPN). Hexokinase and Glucose-6-phosphate Dehydrogenase both serve as a tool in biochemical and analytical studies for determining glucose, fructose, mannose, ATP, and creatin-kinase activity.[3]

Kiszerelés

Package sizes are based on hexokinase units.

Komponensek

Mixture of hexokinase and glucose-6-phosphate dehydrogenase

Egység definíció

Hexokinase Unit Definition: One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C.

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, type N95 (US)

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
0000319468
0000319468
0000296377
0000264626
0000296377

Nem találja a megfelelő verziót?

Ha egy adott verzióra van szüksége, a tétel- vagy cikkszám alapján rákereshet egy adott tanúsítványra.

COA keresése

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Laura Prioretti et al.
Journal of phycology, 52(6), 1094-1102 (2016-10-25)
The sulfate facilitation hypothesis suggests that changes in ocean sulfate concentration influenced the rise to dominance of phytoplankton species of the red lineage. The mechanistic reasons for this phenomenon are not yet understood. We started to address this question by
Fetal bovine serum contains biologically available ATP
Vultaggio-Poma V, et al.
Purinergic Signaling, 20(1), 83-89 (2024)
d-Glucose: Determination with Hexokinase and Glucose-6-phosphate Dehydrogenase
Slein MW
Methods of Enzymatic Analysis, 8(12), 117-130 (1965)
Effect of pH on the stability of hexokinase and glucose 6-phosphate dehydrogenase
Souza M A, et al.
Applied Biochemistry and Biotechnology, 265-272 (2002)
Samantha L Barlow et al.
The Journal of experimental biology, 220(Pt 3), 414-424 (2017-02-06)
Atlantic cod are a commercially important species believed to be threatened by warming seas near their southern, equatorward upper thermal edge of distribution. Limitations to circulatory O

Questions

1–6 of 6 Questions  

  1. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  2. What is the stability of a stock solution of Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast?

    1 answer

    1. Stock solutions prepared in water or 10 mM potassium phosphate buffer pH 8.0 lose approximately 1% activity per month when stored at 4°C.

      Helpful?

  3. What units are the package sizes of Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast, based upon?

    1 answer

    1. This product is packaged on the basis of hexokinase units.

      Helpful?

  4. Can Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast, be used for the determination of glucose?

    1 answer

    1. A method for the determination of glucose using hexokinase and glucose 6-phosphate dehydrogenase is described in "Methods of Enzymatic Analysis" 3rd ed., Vol. VI, p. 163.

      Helpful?

  5. Does Sigma offer any other product which can be used in place of Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast?

    1 answer

    1. Yes. We offer Product No. G3293 (Glucose Assay Reagent), which is a component of the GAHK20 (Glucose Assay Kit).

      Helpful?

  6. How should Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast, be reconstituted?

    1 answer

    1. Prior to determining the activity of this enzyme, Sigma reconstitutes it in 250 mM glycylglycine buffer, pH 7.4.

      Helpful?

Reviews

No rating value

Active Filters

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

Lépjen kapcsolatba a szaktanácsadással