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F7053

Sigma-Aldrich

Anti-Vinculin−FITC antibody, Mouse monoclonal

clone hVIN-1, purified from hybridoma cell culture

Szinonimák:

Monoclonal Anti-Vinculin antibody produced in mouse

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
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About This Item

MDL-szám:
MFCD00162905
UNSPSC kód:
12352203
NACRES:
NA.41

biológiai forrás

mouse

Minőségi szint

200

konjugátum

FITC conjugate

antitest forma

purified immunoglobulin

antitest terméktípus

primary antibodies

klón

hVIN-1, monoclonal

form

buffered aqueous solution

molekulatömeg

antigen 116 kDa

faj reaktivitás

frog, chicken, mouse, canine, human, bovine, rat, turkey

tárolási körülmény

protect from light

technika/technikák

direct immunofluorescence: 1:50 using methanol/acetone fixed cultured bovine MDBK cells

izotípus

IgG1

UniProt elérési szám

P18206

kiszállítva

dry ice

tárolási hőmérséklet

−20°C

célzott transzláció utáni módosítás

unmodified

Géninformáció

human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)

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Egyediség

Specifically labels vinculin at cell-cell and cell-substrate contacts. Reacts strongly with human vinculin. Shows cross-reactivity with smooth muscle metavinculin.

Immunogen

purified human vinculin from uterus.

Alkalmazás

Monoclonal Anti-Vinculin-FITC antibody is suitable for immunofluorescence staining of fibroblasts cells to study the effects of nanofiber spatial organization on cell behaviors. It is also suitable for direct immunofluorescence at a dilution of 1:50 using methanol/acetone fixed cultured bovine MDBK cells.

Biokémiai/fiziológiai hatások

Vinculin is a cytoskeletal protein with molecular mass of ~116 kDa. Vinculin is closely associated with adhesive membrane areas, such as focal contacts, podosomes, or intercellular junctions of the zonula adherens type. It acts as a mediator in interactions of integrins and the actin cytoskeleton. It forms a bridge between integrins, receptors for ECM molecules on the cell surface and the F-actin cytoskeleton. It is associated with talin and α-actinin which directly connects with integrins. It has ability to bind α-catenin as well as the vasodilator stimulated phosphoprotein (VASP) in focal contact assembly.

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Product No.
Leírás
Árazás

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

nwg

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Dokumentumtár megtekintése

Mathieu Maalouf et al.
Nanomaterials (Basel, Switzerland), 12(10) (2022-05-29)
Ultrashort pulse lasers have significant advantages over conventional continuous wave and long pulse lasers for the texturing of metallic surfaces, especially for nanoscale surface structure patterning. Furthermore, ultrafast laser beam polarization allows for the precise control of the spatial alignment
Akiko Hashimoto-Tane et al.
The Journal of experimental medicine, 213(8), 1609-1625 (2016-06-30)
The immunological synapse (IS) formed at the interface between T cells and antigen-presenting cells represents a hallmark of initiation of acquired immunity. T cell activation is initiated at T cell receptor (TCR) microclusters (MCs), in which TCRs and signaling molecules
E E Weiss et al.
The Journal of cell biology, 141(3), 755-764 (1998-06-13)
In epithelial cells, alpha-, beta-, and gamma-catenin are involved in linking the peripheral microfilament belt to the transmembrane protein E-cadherin. alpha-Catenin exhibits sequence homologies over three regions to vinculin, another adherens junction protein. While vinculin is found in cell-matrix and
A Westmeyer et al.
The EMBO journal, 9(7), 2071-2078 (1990-07-01)
We have analyzed the functional domain structure of vinculin, a protein involved in linking microfilaments to the cytoplasmic face of cell membranes in animal cells. For this purpose, we used several monoclonal antibodies raised against chicken gizzard vinculin whose epitopes
Leonel E Rojo et al.
Journal of cosmetic dermatology, 9(3), 185-195 (2010-10-05)
Cell migration, angiogenesis, inflammation, and extracellular matrix remodeling are key events in wound healing. Natural products, including fatty acids (FAs), can accelerate wound healing by modulating the aforementioned events. This study aims to evaluate the effect of lucuma (Pouteria lucuma
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