The recommended storage duration for Fibronectin (F0895) is up to 1 year at 4°C. There has been no testing done on its stability after storage at -80°C or -20°C. A diluted solution of 10ug/mL in PBS can be stored at 4°C for 2-4 weeks. However, the solution cannot be reused to coat culture ware surfaces multiple times; the recommended process involves coating surfaces with a minimal volume, allowing them to air dry for 45 minutes at room temperature, and then storing the cultureware at 4°C for 2-4 weeks.
F0895
Human Fibronectin
FIBRONECTIN FROM HUMAN PLASMA
1 of 1 reviewers received a sample product or took part in a promotion
from human plasma, liquid, 1 mg/mL, suitable for cell culture
Szinonimák:
Fibronectin
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Méret kiválasztása
1 MG
104 000,00 Ft
2 MG
174 000,00 Ft
5 MG
351 000,00 Ft
104 000,00 Ft
Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.
Megrendelés igénylése nagy tételben
Összes fotó(1)
Méret kiválasztása
Nézet módosítása
1 MG
104 000,00 Ft
2 MG
174 000,00 Ft
5 MG
351 000,00 Ft
About This Item
104 000,00 Ft
Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.
Megrendelés igénylése nagy tételben
Javasolt termékek
Terméknév
FIBRONECTIN FROM HUMAN PLASMA, liquid, 0.1% (Solution), BioReagent, suitable for cell culture
biológiai forrás
human plasma
Minőségi szint
sterilitás
sterile
termékcsalád
BioReagent
Forma
liquid
kiszerelés
pkg of 1 mg
pkg of 2 mg
pkg of 5 mg
koncentráció
0.1% (Solution)
technika/technikák
cell culture | mammalian: suitable
felület bevonatolás
1‑5 μg/cm2
oldhatóság
saline: 0.05 M (Tris-buffered)
UniProt elérési szám
Kötési specifikusság
Peptide Source: Collagen
kiszállítva
wet ice
tárolási hőmérséklet
2-8°C
Géninformáció
human ... FN1(2335)
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Általános leírás
Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. The FN1 gene mapped to human chromosome 2q35.[1]
FN occurs as a dimeric protein, linked by two disulfide bonds at the end of C-terminal.[2] Within the fibronectin structure, the domains are compactly arranged close to both the amino and carboxyl terminal of the peptide chains.[3] Moreover, it has been observed that despite arising from a single gene up to 20 distinct variants of this gene have been identified in humans.[2]
FN occurs as a dimeric protein, linked by two disulfide bonds at the end of C-terminal.[2] Within the fibronectin structure, the domains are compactly arranged close to both the amino and carboxyl terminal of the peptide chains.[3] Moreover, it has been observed that despite arising from a single gene up to 20 distinct variants of this gene have been identified in humans.[2]
Alkalmazás
Biokémiai/fiziológiai hatások
Fibronectin function encompasses a diverse range of biological processes including cell adhesion, growth, migration, wound healing, blood coagulation and metastasis.[1] Fibronectin protein plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis. Mutations in FN1 results in glomerulopathy.[8]
Tulajdonságok és előnyök
- Adhesive glycoprotein
- Derived from human plasma
- Recommended surface coverage of 1-5 μg/cm2
Elkészítési megjegyzés
This product is a 0.1% solution of fibronectin. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.
In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C
In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C
Analízis megjegyzés
Protein homogeneity is evaluated by immunoelectrophoresis.
Jogi információk
Sylgard is a registered trademark of The Dow Chemical Company or an affiliated company of Dow
Jogi nyilatkozat
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
kapcsolódó termék
Product No.
Leírás
Árazás
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
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Analitikai tanúsítványok (COA)
Lot/Batch Number
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Az ügyfelek ezeket is megtekintették
Microcontact printing of fibronectin on a biodegradable polymeric surface for skeletal muscle cell orientation.
Altomare L, et al.
The International Journal of Artificial Organs, 33(8), 535-543 (2010)
Repression of KIAA1199 attenuates Wnt-signalling and decreases the proliferation of colon cancer cells.
Birkenkamp-Demtroder K, et al.
British Journal of Cancer, 105(4), 552-552 (2011)
The development of 3-D, in vitro, endothelial culture models for the study of coronary artery disease.
Farcas M A, et al.
BioMedical Engineering OnLine, 8(1), 30-30 (2009)
Jonathan Iu et al.
Tissue engineering. Part A, 23(17-18), 1001-1010 (2017-05-10)
The intervertebral disc (IVD) is composed of nucleus pulposus (NP) surrounded by multilamellated annulus fibrosus (AF), and is located between the vertebral bodies. Current treatments for chronic neck or low back pain do not completely restore the functionality of degenerated
Fibronectin: Molecular Structure, Fibrillar Structure and Mechanochemical Signaling
Dalton C J and Lemmon C A
Cell, 10(9), 2443-2443 (2021)
Cikkek
Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.
Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.
The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
Protocols
Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.
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What is the recommended storage duration for Fibronectin (F0895) at 4°C, -20°C, and -80°C? How long can a diluted solution of 10ug/mL in PBS be stored at 4°C? Is it possible to reuse the Fibronectin solution to coat culture ware surfaces multiple times?
1 answer-
Helpful?
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What is the recommended dilution rate for coating the culture surface with F0895-1MG Fibronectin as per the preparation instructions?
1 answer-
The recommended dilution rate for coating the culture surface with F0895-1MG Fibronectin, as per the preparation instructions, is to start with a concentration in the range of 1 - 5 ug/cm^2. For a 96-well plate well with a surface area of about 0.34 cm^2, approximately 2 ul of F0895 further diluted in saline with a total volume of around 50 ul would be sufficient to cover the bottom of the well.
Helpful?
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Is Product F0895, Fibronectin from human plasma, sterile?
1 answer-
Fibronectin 0.1% solution is a sterile product. It is tested for sterility. The sterility test results can be found on the lot-specific certificate of analysis.
Helpful?
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How much Product F0895, Fibronectin from human plasma, do I use for my application?
1 answer-
Recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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What is the solvent for Product F0895, Fibronectin from human plasma?
1 answer-
This material is solubilized in a 0.05 M Tris-buffered saline, pH 7.5.
Helpful?
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How should I store and handle Product F0895, Fibronectin from human plasma?
1 answer-
Recommended storage of this material is 2-8 °C. Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.
Helpful?
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