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FIBRP-RO

Roche

Fibronectin (pure)

from human plasma, >95% (SDS-PAGE), lyophilized (clear, colorless solution after reconstitution)

Szinonimák:

Fibronectin

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
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About This Item

MDL-szám:
MFCD00131062
UNSPSC kód:
12352202

biológiai forrás

human plasma

Minőségi szint

100

sterilitás

non-sterile; 0.2 μm filtered

Teszt

>95% (SDS-PAGE)

form

lyophilized (clear, colorless solution after reconstitution)

molekulatömeg

440 kDa

kiszerelés

pkg of 1 mg (11051407001)
pkg of 5 mg (11080938001)

gyártó/kereskedő neve

Roche

technika/technikák

cell culture | mammalian: suitable

UniProt elérési szám

P02751

tárolási hőmérséklet

2-8°C

Géninformáció

human ... FN1(2335)

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Általános leírás

Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.

Egyediség

Active on most mammalian cells
Biological activity: Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage).

Alkalmazás

Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes. It has been used for the cell adhesion assay.

Szekvencia

Human plasma fibronectin consists of two similar polypeptide chains ( α-, β-chain, each of 220 kDa), connected by two disulfide bridges.

Fizikai forma

Lyophilizate containing 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin

Elkészítési megjegyzés

Working concentration: 5μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.

Feloldás

Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.

Egyéb megjegyzések

For life science research only. Not for use in diagnostic procedures.

Jogi nyilatkozat

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 1

Lobbanási pont (F)

does not flash

Lobbanási pont (C)

does not flash

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Roberta Bulla et al.
Nature communications, 7, 10346-10346 (2016-02-03)
Complement C1q is the activator of the classical pathway. However, it is now recognized that C1q can exert functions unrelated to complement activation. Here we show that C1q, but not C4, is expressed in the stroma and vascular endothelium of
Anindita Dutta et al.
International journal of clinical and experimental pathology, 3(3), 288-302 (2010-03-13)
The interaction of cells with adhesion proteins in the extracellular matrix (ECM) provides signals which affect the morphology, motility, gene expression and survival of adherent cells. In the present communication we cultured K562 cells in presence of fibronectin to study
Fibronectin at a glance.
Roumen Pankov et al.
Journal of cell science, 115(Pt 20), 3861-3863 (2002-09-24)
Benjamin N Rollo et al.
F1000Research, 4, 113-113 (2015-06-13)
The avian enteric nervous system (ENS) consists of a vast number of unusually small ganglia compared to other peripheral ganglia. Each ENS ganglion at mid-gestation has a core of neurons and a shell of mesenchymal precursor/glia-like enteric neural crest (ENC)

Cikkek

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

Protocols

Fibronectin Coating Protocol

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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