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10838039001

Roche

Fibronectin

from human plasma

Szinonimák:

Fibronectin

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)

About This Item

MDL-szám:
MFCD00131062
UNSPSC kód:
12352207

biológiai forrás

human plasma

Minőségi szint

100

sterilitás

non-sterile; 0.2 μm filtered

Teszt

95% (SDS-PAGE)

Forma

lyophilized (clear, colorless solution after reconstitution)

molekulatömeg

440 kDa

kiszerelés

pkg of 5 mg

gyártó/kereskedő neve

Roche

tárolási körülmény

avoid repeated freeze/thaw cycles

technika/technikák

cell culture | mammalian: suitable

UniProt elérési szám

P02751

tárolási hőmérséklet

2-8°C

Géninformáció

human ... FN1(2335)

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Általános leírás

Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.

Egyediség

Active on most mammalian cells

Alkalmazás

Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes.

Tesztelési mód

Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage)

Fizikai forma

Lyophilizate (contains 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin), before lyophilization filtered through a 0.2 μm pore size membrane.

Elkészítési megjegyzés

Working concentration: 5 μg/cm2
5 μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.

Feloldás

Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate for 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.

Egyéb megjegyzések

For life science research only. Not for use in diagnostic procedures.

Jogi nyilatkozat

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 1

Lobbanási pont (F)

does not flash

Lobbanási pont (C)

does not flash

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Analitikai tanúsítványok (COA)

Lot/Batch Number
72438200
67434100
66091400
60026000
57114400

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Cikkek

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

Protocols

Fibronectin Coating Protocol

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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