ABT1487
Anti-alpha-Actin Antibody, Smooth Muscle
from rabbit, purified by affinity chromatography
Szinonimák:
Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(7)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
antitest forma
affinity isolated antibody
antitest terméktípus
primary antibodies
klón
polyclonal
tisztítva
affinity chromatography
faj reaktivitás
rat, mouse, human
faj reaktivitás (homológia által előrejelzett)
chicken (based on 100% sequence homology)
technika/technikák
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI elérési szám
UniProt elérési szám
kiszállítva
wet ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... ACTA1(58)
Általános leírás
Actin, aortic smooth muscle (UniProt P62736; also known as Alpha-actin-2, Cell growth-inhibiting gene 46 protein) is encoded by the ACTA2 (also known as AAT6, ACTSA, ACTVS, GIG46, MYMY5) gene (Gene ID 59) in human. Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin is encoded by the ACTA1 gene, smooth muscle alpha-actin by the ACTA2 gene, cytoplasmic beta-actin by the ACTB gene, cardiac muscle alpha-actin by the ACTC1 gene, cytoplasmic gamma-actin by the ACTG1 gene, and smooth muscle gamma-actin by the ACTG2 (a.k.a. ACTA3) gene. Although actins show >90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic β and γ-actins, are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt.
Egyediség
This polyclonal antibody targets the N-terminal sequence specific to smooth muscle alpha-actin (ACTA2), but not skeletal muscle alpha-actin (ACTA1), cytoplasmic beta-actin (ACTB), cardiac muscle alpha-actin (ACTC1), cytoplasmic gamma-actin (ACTG1), or smooth muscle gamma-actin (ACTG2; ACTA3).
Immunogen
Epitope: N-terminus.
KLH-conjugated linear peptide corresponding to the N-terminual sequence of human smooth muscle alpha-actin.
Alkalmazás
Anti-alpha-Actin Antibody, Smooth Muscle is an antibody against alpha-Actin for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation.
Immunocytochemistry Analysis: 5.0 µg/mL from a representative lot immunostained smooth muscle alpha-Actin in NIH/3T3 fibroblasts, HUVECs, A431 and HeLa cells.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot immunostained smooth muscle cells in human uterus tissue sections.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated smooth muscle alpha-Actin from 500 µg of C2C12 mouse myoblast lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot immunostained smooth muscle cells in human uterus tissue sections.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated smooth muscle alpha-Actin from 500 µg of C2C12 mouse myoblast lysate.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Minőség
Evaluated by Western Blotting in C2C12 mouse myoblast lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected smooth muscle alpha-Actin in 10 µg of C2C12 mouse myoblast lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected smooth muscle alpha-Actin in 10 µg of C2C12 mouse myoblast lysate.
Cél megnevezése
~42 kDa observed.
Fizikai forma
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Tárolás és stabilitás
Stable for 1 year at 2-8°C from date of receipt.
Egyéb megjegyzések
Concentration: Please refer to lot specific datasheet.
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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