A2228

Monoclonal Anti-β-Actin antibody produced in mouse

clone AC-74, purified immunoglobulin, buffered aqueous solution

• Szinonimák: Actin Antibody Sigma, Beta Actin Antibody - Monoclonal Anti-β-Actin antibody produced in mouse, Beta Actin Antibody Sigma, Beta Actin Sigma Antibody, Sigma Actin Antibody, Sigma Beta Actin Antibody
• MDL-szám: MFCD00164531
• UNSPSC kód: 12352203
• NACRES: NA.41
• konjugátum: unconjugated
• application: ARR; ICC; IHC; WB
• klón: AC-74, monoclonal
• faj reaktivitás: rabbit, sheep, cat, guinea pig, bovine, mouse, chicken, wide range, Hirudo medicinalis, canine, Drosophila, carp, pig, rat, human
• citations: 2972
• technika/technikák: immunocytochemistry: 10-40 μg/mL using human or chicken fibroblasts; immunohistochemistry: suitable; microarray: suitable; western blot: 0.5-1 μg/mL using total cell extracts of human or chicken fibroblasts

Tulajdonságok

• biológiai forrás: mouse
• Minőségi szint: 300
• konjugátum: unconjugated
• antitest forma: purified immunoglobulin
• antitest terméktípus: primary antibodies
• klón: AC-74, monoclonal
• Forma: buffered aqueous solution
• molekulatömeg: antigen 42 kDa
• faj reaktivitás: rabbit, sheep, cat, guinea pig, bovine, mouse, chicken, wide range, Hirudo medicinalis, canine, Drosophila, carp, pig, rat, human
• nem léphet reakcióba: Dictyostelium discoideum
• koncentráció: ~2 mg/mL
• technika/technikák: immunocytochemistry: 10-40 μg/mL using human or chicken fibroblasts; immunohistochemistry: suitable; microarray: suitable; western blot: 0.5-1 μg/mL using total cell extracts of human or chicken fibroblasts
• izotípus: IgG2a
• UniProt elérési szám: P60709
• alkalmazás(ok): research pathology
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... ACTB(60) ; mouse ... Actb(11461) ; rat ... Actb(81822)

Leírás

Általános leírás

ACTB (actin β) gene codes for β-actin. It is a cytoskeletal housekeeping protein. It is present in the cytoplasm. This gene is located on human chromosome 7p22.1.

Recognizes an epitope located on the N-terminal end of the β-isoform of actin. It specifically labels β-actin in a wide variety of tissues and species. In immunofluorescent staining of chicken gizzard ultrathin cryosections, the antibody labels the dense bodies, the longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. The antibody does not react with adult cardiac or skeletal muscle (besides traces due to contamination of the sample with non-muscle cells) or with β-actin expressing cells in Dictyostelium discoideum amoeba. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. B5, methacarn, ethanol or Bouin′s solutions may be also used as fixatives. The antibody can be used as probes for β-actin as an internal control in immunoblotting.

Immunogén

slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Alkalmazás

Monoclonal Anti-β-Actin antibody has been used in western blot and two-dimensional gel immunoblot.

Monoclonal anti-β-actin antibody can be used for immunocytochemistry (10-40μg/mL using human or chicken fibroblasts), immunohistochemistry and microarray analysis.

Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.

Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody.

Biokémiai/fiziológiai hatások

Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin.
β-Actin modulates cell growth, migration and the G-actin pool. Mutations in ACTB leads to pleiotropic developmental disorder.

Fizikai forma

Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Tárolás és stabilitás

Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Egyéb megjegyzések

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)