199303

Resazurin sodium salt

certified by the Biological Stain Commission

• Szinonimák: 7-Hydroxy-3H-phenoxazin-3-one-10-oxide sodium salt
• Tapasztalati képlet (Hill-képlet): C₁₂H₆NNaO₄
• CAS-szám: 62758-13-8
• Molekulatömeg: 251.17
• Beilstein: 3794971
• EC-szám: 263-718-5
• MDL-szám: MFCD00005036
• UNSPSC kód: 12171500
• PubChem Substance ID: 24851905
• NACRES: NA.47

Tulajdonságok

• grade: certified by the Biological Stain Commission
• Minőségi szint: 200
• Forma: powder
• összetétel: Dye content, 75%
• oldhatóság: 1 M NH4OH: 1 mg/mL
• λmax: 380 nm (2nd); 598 nm
• alkalmazás(ok): diagnostic assay manufacturing; hematology; histology
• tárolási hőmérséklet: room temp
• SMILES string: [Na+].[O-]c1ccc2c(OC3=CC(=O)C=CC3=[N+]2[O-])c1
• InChI: 1S/C12H7NO4.Na/c14-7-1-3-9-11(5-7)17-12-6-8(15)2-4-10(12)13(9)16;/h1-6,14H;/q;+1/p-1
• Nemzetközi kémiai azonosító kulcs: IVGPGQSSDLDOLH-UHFFFAOYSA-M

Leírás

Általános leírás

Resazurin is a small N-oxide derivative of dihydroxyphenoxazone. It is a non-ionic dye containing a small and planar chromophore. It is a redox indicator and changes from bluish violet through pink to colorless upon reduction. It is also used as a pH indicator for Ph 3.5 to 6.5 wherein it changed from orange to bluish violet. It is commercially available as resazurin sodium salt, which is also known as 3H-Phenoxazin-3-one, 7-hydroxy-, 10-oxide, sodium salt. It forms the red, fluorescent hydroxyphenoxazone resorufin on reduction and colorless hydroresorufin on further reduction.

Alkalmazás

Resazurin is mostly used as a biological redox indicator. The bioreduction of the dye reduces the amount of the oxidized form (blue) and concomitantly increases the fluorescent intermediate (red). Its applications include:

• the measurement of metabolic activity and the proliferation of living cells
• the detection of bacterial reduction in milk
• in viability assays as a component of culture media for the detection of viable bacteria
• to determine the number of motile sperm in sheep semen
• to assay lactate dehydrogenase
• as an analytical reagent to quantify L-glutamate

For the measurement of metabolic activity and proliferation of living cells. The bioreduction of the dye reduces the amount of the oxidized form (blue) and concomitantly increases the fluorescent intermediate (red).

Biztonsági adatok

• Piktogramok: GHS07
• Figyelmeztetés: Warning
• Figyelmeztető mondatok: H315,H319,H335
• Óvintézkedésre vonatkozó mondatok: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Veszélyességi osztályok: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Célzott szervek: Respiratory system
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: dust mask type N95 (US), Eyeshields, Gloves