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D0819

DMEM - high glucose

StableCell™, with stable glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

Medio de Eagle modificado de Dulbecco, glucosa elevada, DME, DMEM

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UNSPSC Code:
12352207
NACRES:
NA.75
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Nombre del producto

Dulbecco′s Modified Eagle′s Medium, With 4500 mg/L glucose, stable glutamine, and sodium bicarbonate, without sodium pyruvate., liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

suitability

suitable for

components

stable glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: no
glucose: high
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

General description

This DMEM-Hi glucose medium differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.

Application

El medio de Eagle modificado de Dulbecco (DMEM) es una modificación del medio basal Eagle (BME) que contiene el cuádruple de concentraciones de aminoácidos y vitaminas. La formulación original contenía 1000 mg/l de glucosa y se utilizaba para cultivar células embrionarias de ratón. Desde entonces, se ha modificado de varias formas para soportar cultivos primarios de células de ratón y de pollo, así como una variedad de células normales y transformadas. Cada uno de estos medios ofrece una combinación diferente de L-glutamina y piruvato sódico. Además, los niveles de glucosa se han elevado a 4 500 mg/l, lo que contribuye al nombre «DMEM/High».

Legal Information

StableCell is a trademark of Sigma-Aldrich Co. LLC

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Este artículo
D0822D0697FG0435
StableCell™ DMEM - high glucose With 4500 mg/L glucose, stable glutamine, and sodium bicarbonate, without sodium pyruvate., liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

D0819

StableCell™ DMEM - high glucose

StableCell™ DMEM - high glucose With 4500 mg/L glucose, stable glutamine, sodium pyruvate and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

D0822

StableCell™ DMEM - high glucose

StableCell™ DMEM/F12 With stable glutamine, 15mM HEPES and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

D0697

StableCell™ DMEM/F12

Sigma-Aldrich

Sigma-Aldrich

FG0435

Dulbecco′s Modified Eagle′s Medium - high glucose

form

liquid

form

liquid

form

liquid

form

liquid

suitability

suitable for

suitability

-

suitability

-

suitability

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

components

stable glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: no
glucose: high
NaHCO3: yes

components

glucose: high
HEPES: no
stable glutamine
sodium pyruvate: yes
phenol red: yes
NaHCO3: yes

components

glucose: high
sodium pyruvate: yes
stable glutamine: yes
NaHCO3: yes
phenol red: yes
HEPES: yes

components

phenol red: yes
glucose: high
stable glutamine: yes
sodium pyruvate: no
HEPES: no
NaHCO3: yes

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

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Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
RNBL7478
RNBL4169
RNBL0667
RNBK9594
RNBK8721

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SKUGTIN
D0819-500ML04061833558140

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