HPLC Columns
Supelco® HPLC and UHPLC columns meet today’s demands of fast U/HPLC, LC-MS, and biopolymer separation, as well as regulated pharmacopeia and agency methods within various industries. Supelco® HPLC products are available with Fused-Core® particle technology, monolithic silica, and fully porous particles, including ultra-pure silica. In addition, porous graphitic carbon (PGC) particles, and polymeric particles are available for high pH stability.
Section Overview
- Porous Graphitic Carbon (PGC) Particles
- Superficially Porous Silica Particles (SPP)/Fused Core® Technology
- Monolithic Silica
- Fully Porous Silica Particles (FPP) Type B (High Purity Silica)
- Fully Porous Silica Particles (FPP) Type A (Conventional Silica)
- Fully Porous Polymeric Particles
- Chiral HPLC Columns
Porous graphitic carbon (PGC) particles
Porous graphitic carbon (PGC) columns, like the Supel™ Carbon LC column, provide unique chromatographic properties that are unmatched by silica particle-packed columns. The full pH range can be used as well as high temperature (> 200 °C), opening up new frontiers for chromatographers.
Superficially porous silica particles (SPP)/Fused Core® Technology
Superficially porous silica columns deliver higher speed and efficiency than fully porous particles of the same size. Fused-Core® HPLC columns like Ascentis® Express and BIOshell™ can convert any HPLC system into a fast HPLC workhorse with maximum speed and performance.
Monolithic silica
- Monolithic, silica-based HPLC columns such as Chromolith® HPLC columns allow rapid separations at very low column backpressure with high matrix tolerance and extended column lifetime, thus making them ideal products for high throughput and cost-efficient analyses of matrix rich samples.
- Chromolith® HPLC columns are made from monolithic silica with a bimodal pore structure having 2 µm macropores and 13 nm mesopores. This structure leads to various key advantages to the column.
- Chromolith® WP columns are wide pore (300 Å) monolithic silica columns made up of a single continuous rod of high purity, porous silica. These columns have shown great potential compared to conventional columns with consistent performance over a longer lifetime. In addition to various RP phases, a Protein A affinity column and an epoxy column, that enable customer specific modifications, are also available.
Fully porous silica particles (FPP) Type B (high purity silica)
- Purospher® STAR HPLC and UHPLC columns are best suited for the peak-tailing free separation of basic, neutral, and metal chelating samples. Their superior performance and stability allow maximum flexibility in method development with extended pH stability.
- Discovery® HPLC columns are efficient for the analysis of biomolecules and small molecules. These columns have polar and non-polar bondings, with unique retention, selectivity, and no-bleed properties for LC-MS applications.
- The Ascentis® bonded phases have a wide range of selectivity. Type B silica makes the column inert, stable, and reproducible. The high surface area of the silica is suitable for preparative loading capacity.
- SeQuant® HILIC HPLC columns (hydrophilic interaction liquid chromatography) carry densely bonded, zwitterionic functional groups. These HILIC columns are available in a variety of lengths, particle sizes, and pore dimensions while showing an exceptional reproducibility and robustness.
- Sepax® HPLC columns are highly porous silica-based columns mainly utilized for peptides and protein analysis.
Fully porous silica particles (FPP) Type A (conventional silica)
- Supelcosil® silica-based HPLC column line has a wide range of bonded phase chemistries, with the particle sizes and column configurations ranging from microbore to preparative scale. These columns show great efficiency for polar, nonpolar, and charged analytes.
- LiChrospher® and Superspher® HPLC columns are highly versatile and reliable, proven in thousands of established HPLC methods.
- LiChrosorb® HPLC columns are packed with irregular silica particles ranging from analytical scale 5 µm to large particles sized for production scale.
- TSKgel® HPLC and UHPLC columns are available for gel filtration, gel permeation, ion exchange, reversed-phase, hydrophobic interaction, hydrophilic interaction, and affinity chromatography. (only available in the US and Europe)
Fully porous polymeric particles
- apHera™ reversed-phase and amino HPLC columns are stable at the pH range 2-12 and are amenable to washing with alkaline solutions. They are ideal for the analysis of peptides, small proteins, carbohydrates, polar drugs, and organic acids.
- Supelcogel® HPLC columns are packed with polystyrene-divinylbenzene co-polymer (PS-DVB) making them chemically stable. The two variations of PS-DVB co-polymer (6% and 8% cross-linked) maximize the separation process of the samples.
- Hamilton Polymeric Reversed Phase HPLC columns are extremely inert, efficient, and durable, with high mechanical and thermal stability.
- TSKgel® HPLC and UHPLC columns are available for the separation of proteins, peptides, and other biomolecules. The columns containing silica and methacrylate particles with different pore sizes can accommodate analytes with a wide range of molecular weights. (only available in the US and Europe)
Chiral HPLC Columns
We have a wide range of chiral HPLC columns for various applications.
- Astec CYCLOBOND™ Chiral columns have bonded cyclodextrin phases that allow selective chemical separation of organic molecules.
- Astec CHIROBIOTICTM Chiral columns are based on macrocyclic glycoproteins bonded to high purity silica gels available for LC-MS chiral applications.
- Astec Cellulose DMP Chiral columns with spherical, high-purity porous silica coated with DMPC (3,5-dimethylphenyl carbamate); can separate a wide range of chiral compounds under normal phase, polar organic, and SFC conditions.
- Astec Copper Ligand Exchange Chiral columns are beneficial for resolving hydroxy acids, amino acids, bi-functional racemates, and other amines.
Related Resources
- Flyer: Ascentis®Express PCS-C18, 2.7 µm U/HPLC Columns
The Ascentis® Express PCS-C18 columns are designed for effective separation of basic, acidic, or neutral compounds using low ionic strength (formic acid) mobile phase conditions.
- Brochure: HPLC Column Selection Guide
The comprehensive Supelco® portfolio of analytical solutions to meet your U/HPLC and LC-MS needs.
- Brochure: HPLC Method Development Guide
High performance liquid chromatography (HPLC) is a technique for separating analytes dissolved in a liquid, mobile phase by using their specific interaction with a stationary phase (chromatography column).
- Brochure: Ascentis® Express Columns
Based on Fused-Core® particle technology, Ascentis® Express columns provide a exceptional advancements in HPLC column performance and the benefits of high sample throughput at maximum resolution.
- Brochure: BIOshell™ Columns
The BIOshell™ line of superficially porous particle (SPP) packed columns has been developed to aid research into understanding the subtleties of the molecule that is being developed.
- Brochure: Chromolith® HPLC Columns
To truly accelerate chromatographic separations, there is no better choice than Chromolith® HPLC columns. Due to their revolutionary, monolithic technology, Chromolith® columns provide excellent and rapid separations with extremely high robustness and matrix tolerance compared to particulate columns.
- Brochure: Purospher STAR Columns
The success of any method depends on the quality of the stationary phase. Precise, long-term reproducibility is a key factor in achieving reliable results. The base silica of Purospher™ STAR columns is 99.999% pure.
- Brochure: Chiral HPLC Columns
Columns for versatile and robust Chiral HPLC and LC-MS separations.
- Brochure: HPLC Accessories
The use of HPLC systems to achieve faster analyses and increased resolution pushes systems toward higher operating pressures. For instance, faster flow rates, longer column lengths, and reduced particle sizes gain a performance advantage in HPLC, but at a cost of increased operating pressures.
- Brochure: Discovery Bio Columns
Many of the challenges facing researchers in the proteomics and biopharmaceutical fields are related to the need to obtain as much information as possible on very limited samples. We designed Discovery® BIO Wide Pore reversed-phase HPLC columns to address these challenges.
- Brochure: LC-MS Resource Guide
In this guide, we provide a premier selection of proven Supelco® tools and consumables that meet the requirements of scientists who primarily use the LC-MS technique for analysis of drugs, biomolecules separation and analytical assays.
- Brochure: LC-MS Contaminants
In this technical bulletin, you will learn some tips on identifying LC-MS contaminants and avoiding contamination. Our 110 years of separation expertise, combined with our precision-manufactured products, give you the greatest chance of obtaining reproducible, clean data.
- Application Note: Supelco® HPLC Products: The Best Choice for Any LC Instrument
Since the 1970’s, Supelco® has been a trusted name in high performance liquid chromatography (HPLC) consumables. From a diverse array of columns packed with different stationary phases to a complete portfolio of HPLC accessories, the Supelco® portfolio of HPLC consumables caters to the needs of all analysts.
- Application Guide: Drug of Abuse
Application compilation with focus on the LC-MS analysis of drugs of abuse in biological samples.
- HPLC Method Transfer Calculator
Easily transfer a method between HPLC columns, (scale between microbore through preparative range), based on the 2 column dimensions and current method conditions.
- Wall Chart: HPLC Packings for USP Compendial Methods
The official pharmaceutical analysis monographs in the United States Pharmacopeia (USP) detail the methods used by pharmaceutical manufacturers for quality control of bulk drug substances and dosage form preparations.
- Application Guide: Ascentis® Express and BIOshell™ Column Cleaning Procedures and Best Practices
To maximize the lifetime of an HPLC column and ensure its top performance, it is necessary to properly maintain the column. Many different factors can contribute to loss of resolution or sensitivity, but often times the following factors lead to the vast majority of column performance issues.
Related Webinars
In this webcast, the origins and problems in LC–MS arising from impurities and contaminations will be described in detail and tips and tricks will be provided in order to enable or maintain high quality and high sensitivity LC-MS analyses.
Learn about porous graphitic carbon, a stationary phase material that is fully porous, carbon-based material—it is capable of analyzing small molecules and smaller proteins and peptides.
Quantitation of monoclonal antibodies by LC-MS is a difficult analytical challenge due to the complexity in the workflow, from sample prep to LC-MS quantitation. This presentation will highlight a simple and high throughput method for the quantitation of monoclonal antibodies and proteins in human and animal serum.
The focus of this seminar is to provide an overview of different strategies and methods employed over the past several years in characterizing samples containing chiral compounds.
This presentation focuses on recent advances, in the past two years, in 'carbon-HPLC' in the analysis of compounds classically associated with being difficult to separate by conventional 'silica-HPLC.'
Join our webinar focusing on "How to Increase The Cost Effectiveness for HPLC Separations of Challenging and Matrix-rich Samples" for the potential for cost and time savings by increasing lab productivity with faster separations and column materials providing very high matrix tolerance.
*Tosoh Bioscience columns are available in the following countries:
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