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N3786

Sigma-Aldrich

α(2→3,6,8,9) Neuraminidase aus Arthrobacter ureafaciens

Proteomics Grade, suitable for MALDI-TOF MS

Synonym(e):

Neuraminidase aus Arthrobacter ureafaciens, Acyl-Neuraminyl-Hydrolase, Sialidase

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About This Item

CAS-Nummer:
9001-67-6
EC-Nummer:
3.2.1.18 (BRENDA, IUBMB)
EG-Nummer:
232-624-6
MDL-Nummer:
MFCD00131711
UNSPSC-Code:
12352204
NACRES:
NA.32

Form

lyophilized powder

Qualitätsniveau

200

Qualität

Proteomics Grade

Spezifische Aktivität

≥25 U/vial

Eignung

suitable for MALDI-TOF MS

Lagertemp.

2-8°C

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Anwendung

Neuraminidase is an important deglycosylation enzyme capable of cleaving all non-reducing unbranched N-acetylneuraminic and N-glycolylneuraminic acid residues by hydrolysis of α(2→6), α(2→3), α(2→8), and α(2→9) linkages (affinity in the order given). Branched sialic acids may also be cleaved with the use of high concentrations of enzyme and prolonged incubations. Desialylated glycoproteins may then be further characterized by treatment with various exoglycosidases resulting in partial or complete O-deglycosylation. SDS-PAGE and MALDI-TOF MS are typically utilized in purification, structural analysis, and sequencing process. These techniques also remove heterogeneity and charge from the glycoprotein.

Verpackung

Provided with 5× concentrate reaction buffer.

Qualität

The buffer solution has been specifically formulated to assure maximum enzyme stability and assay sensitivity.

Einheitendefinition

One unit will release 1 nmole of 4-methylumbelliferone from 2-(4-methylumbelliferyl) α-D-N-acetylneuraminic acid per minute at pH 5.5 at 37° C.

Physikalische Form

Lyophilized powder

Kit-Komponenten auch einzeln erhältlich

Produkt-Nr.
Beschreibung
SDB
  • N3536Neuraminidase 5X Reaction Buffer 1.5 mLSDB

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Signalwort

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Lagerklassenschlüssel

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