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Key Documents

93017

Sigma-Aldrich

SSC Buffer 20x

for Northern and Southern blotting, solution

Sinônimo(s):

20x SSC

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About This Item

Código UNSPSC:
12161700
NACRES:
NA.25

grau

for molecular biology

Nível de qualidade

200

linha de produto

BioReagent

forma

solution

técnica(s)

Southern blotting: suitable

Impurezas

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

pH

7.0±0.2

densidade

1.16 g/mL at 20 °C

temperatura de armazenamento

room temp

Descrição geral

SSC Buffer 20x Concentrate is a standard reagent in Southern and Northern blotting procedures. This concentrated formula can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.

Aplicação

Suitable for:
  • Preparing nucleic acids for hybridization
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for Northern and Southern blots

Características e benefícios

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations
  • Easily diluted to any concentration needed

Componentes

SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

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Nº do produto
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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves

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Protocolos

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Conteúdo relacionado

Water for Nucleic Acid Gel Electrophoresis

Learn how water impurities can affect DNA and RNA electrophoresis and blotting. High purity water purified using ultrafiltration is suitable for nucleic acid electrophoresis, Northern and Southern blotting.

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