T9285

Tris-EDTA buffer solution

100X concentrate for Northern and Southern blotting

• Sinônimo(s): Tris-EDTA wash buffer, TE buffer solution
• Número MDL: MFCD00236359
• Código UNSPSC: 41105319
• ID de substância PubChem: 329826904
• NACRES: NA.25

Propriedades

• grau: for molecular biology
• Nível de qualidade: 200
• esterilidade: 0.2 μm filtered
• forma: liquid
• pH: 7.8-8.2 (1 × in water)
• aplicação(ões): sample preparation
• atividade externa: DNase, RNase, none detected
• temperatura de armazenamento: room temp
• cadeia de caracteres SMILES: NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
• chave InChI: VLEIUWBSEKKKFX-UHFFFAOYSA-N

Descrição

Descrição geral

Tris EDTA buffer is commonly used in molecular biology for reconstitution and storage of many precipitated biomolecules, including DNA and RNA. Tris-HCl is a commonly used buffer and EDTA is a metal chelating agent. TE buffer solubilizes nucleic acids while protecting the molecules from degradation. It is used in nucleic acid isolation, which may be done prior to nucleic acid gel electrophoresis and Northern or Southern blot hybridization.

Aplicação

TE buffer has been used for:

• isolate, resuspend, or store DNA and RNA for PCR or qPCR
• gel electrophoresis, including Northern and Southern blotting
• bacterial cells, for AmpC disk test, to measure β-lactamases released from the cells
• antigen retrieval for immunohistochemistry

Componentes

TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.

Outras notas

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Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable