This product has been tested successfully at 2 µL, 5µL, and 7 µL., however for general use, a starting volume of 5 µL is recommended. Dilute 5 µL volume of D0428 with 25 µL of gel loading buffer. Product G2526 is used for testing purposes. For best results, a 0.75% gel is recommended.
See the link below to Gel Loading Buffer, G2526:
https://www.sigmaaldrich.com/product/sigma/g2526
D0428
1 kb DNA Ladder
for DNA electrophoresis
Sinônimo(s):
1kb gel ladder, 1kb gel marker, 1kb ladder for gel electrophoresis, DNA gel marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker
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1 VIAL
R$ 1.061,00
R$ 1.061,00
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1 VIAL
R$ 1.061,00
About This Item
Código UNSPSC:
41105335
NACRES:
NA.25
R$ 1.061,00
Em estoqueDetalhes
Produtos recomendados
Nível de qualidade
100
200
Formulário
liquid
adequação
suitable for electrophoresis (DNA)
temperatura de armazenamento
−20°C
Descrição geral
Sigma′s 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. In nucleic acid gel electrophoresis, five μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting. All DNA markers can be stained with ethidium bromide, SYBR® Green, and Nancy-520.
Aplicação
1 kb DNA Ladder has been used as a molecular marker to determine the molecular weight and size of double stranded DNA during gel electrophoresis.[1]
Componentes
Sigma′s 1kb Ladder is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
Outras notas
For optimal resolution, the recommended agarose gel concentration is 0.75%.
Informações legais
SYBR is a registered trademark of Life Technologies
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Código de classe de armazenamento
10 - Combustible liquids
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Artigos
Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
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how to use this product. in what ratio we have to add dye in this.
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