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Key Documents

S0902

Sigma-Aldrich

SSC Buffer

for Northern and Southern blotting, powder blend

Sinônimo(s):

Saline-sodium citrate buffer

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About This Item

Código UNSPSC:
41105319
NACRES:
NA.25

Nível de qualidade

200

forma

powder blend

pH

6.8-7.2 (20-25 °C, 263 mg/L in water)

solubilidade

water: 263 mg/mL, clear, colorless

atividade externa

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

temperatura de armazenamento

room temp

Descrição geral

SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.

Aplicação

Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique. It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer.
Suitable for:
  • Preparing nucleic acids for hybridization
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for Northern and Southern blots

Características e benefícios

  • Multi-purpose use in hybridizations
  • Easily reconstituted and diluted to any concentration needed

Componentes

Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

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Código de classe de armazenamento

13 - Non Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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M Böttger et al.
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Complexes of histones H1 with superhelical SV40 DNA obtained by direct mixing were studied in 0.1 SSC buffer corresponding to 0.02 M Na+. Depending on the molar input ratio H1/DNA three classes of sedimenting species were observed: (1) a component
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Protocolos

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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