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S6639

Sigma-Aldrich

SSC Buffer 20× Concentrate

for Northern and Southern blotting, solution

Sinônimo(s):

20X SSC Buffer, SSC hybridization buffer, Saline-sodium citrate buffer

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About This Item

Código UNSPSC:
41105319
NACRES:
NA.25

grau

for molecular biology

Nível de qualidade

200

descrição

Saline-Sodium Citrate buffer, made with ultrapure water

esterilidade

0.2 μm filtered

forma

solution

aplicação(ões)

diagnostic assay manufacturing

atividade externa

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

temperatura de armazenamento

room temp

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Descrição geral

SSC (Saline Sodium Citrate) Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization.

Aplicação

SSC Buffer 20× Concentrate has been used
  • as a hybridization buffer on in situ hybridization human-specific repetitive Alu sequence
  • as a washing buffer for membrane for Southern hybridization, dot blot of mice genomic DNA and northern blotting of microRNA
  • as an incubation buffer in fluorescence in situ hybridization (FISH) of L1 retrotransposon

Características e benefícios

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations

Componentes

SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

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Chronic hypoxia facilitates Alzheimer's disease through demethylation of gamma-secretase by downregulating DNA methyltransferase 3b
Liu H, et al.
Alzheimers Dement., 12(2), 130-143 (2016)
L1 retrotransposon expression in circulating tumor cells
Papasotiriou I, et al.
Testing, 12(2), e0171466-e0171466 (2017)
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PLoS biology, 18(11), e3000675-e3000675 (2020-11-21)
Changes in cell identities and positions underlie tissue development and disease progression. Although single-cell mRNA sequencing (scRNA-Seq) methods rapidly generate extensive lists of cell states, spatially resolved single-cell mapping presents a challenging task. We developed SCRINSHOT (Single-Cell Resolution IN Situ
Chang-Gong Liu et al.
Methods (San Diego, Calif.), 44(1), 22-30 (2007-12-26)
After 12 years from its first application, microarray technology has become the reference technique to monitor gene expression of thousands of genes in the same experiment. In the past few years an increasing amount of evidence showed the importance of
M Böttger et al.
Nucleic acids research, 9(20), 5253-5268 (1981-10-24)
Complexes of histones H1 with superhelical SV40 DNA obtained by direct mixing were studied in 0.1 SSC buffer corresponding to 0.02 M Na+. Depending on the molar input ratio H1/DNA three classes of sedimenting species were observed: (1) a component
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