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D2532

Sigma-Aldrich

Denhardt′s Solution 50x

for Northern and Southern blotting, solution

Sinônimo(s):

blocking reagent, blocking solution, denhardts, hybridization buffer, hybridization solution, northern blocking solution, pre-hybridization, southern blocking solution

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About This Item

Código UNSPSC:
12161700
NACRES:
NA.25

forma

solution

técnica(s)

Southern blotting: suitable

atividade externa

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

temperatura de armazenamento

−20°C

Categorias relacionadas

Descrição geral

Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This 50x concentrate can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Componentes

Denhardt′s Solution is 1% Bovine Serum Albumin (BSA), 1 % Ficoll, and 1 % Polyvinylpyrrolidone (PVP) in water.

produto relacionado

Nº do produto
Descrição
Preços

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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E J Mackie et al.
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