Pular para o conteúdo
Merck
Todas as fotos(1)

Documentos

H7140

Sigma-Aldrich

Hybridization Solution

for Northern and Southern blotting, liquid (2x concentrate)

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(1)

About This Item

Código UNSPSC:
12161700
NACRES:
NA.25

Nível de qualidade

100

forma

liquid (2x concentrate)

atividade externa

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

temperatura de armazenamento

−20°C

Categorias relacionadas

Descrição geral

Sigma′s Hybridization Solution is a 2x ready-to-use hybridization buffer for Northern and Southern blot protocols.

Aplicação

Hybridization Solution has been used to hybridise the nitrocellulose membrane. It has also been used in DNA hybridization.
Suitable for use as a hybridization buffer in Northern and Southern blot protocols.

Componentes

Sigma′s Hybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt′s solutions:
200 μg/mL DNA
2mg Bovine Serum Albumin (BSA)
2mg Ficoll
2mg Polyvinylpyrrolidone (PVP)
0.15 M sodium citrate
1.5 M NaCl

produto relacionado

Nº do produto
Descrição
Preços

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Os clientes também visualizaram

Slide 1 of 4

1 of 4

Neutralizing Buffer for Northern and Southern blotting, powder

Sigma-Aldrich

N6019

Neutralizing Buffer

Denhardt’s Solution for Northern and Southern blotting, lyophilized powder

Sigma-Aldrich

D9905

Denhardt′s Solution

Bionic™ Buffer, 10X Concentrate for Northern and Southern blotting, solution

Sigma-Aldrich

B6185

Bionic™ Buffer, 10X Concentrate

Sigma-Aldrich

Sigma-Aldrich

S0902

SSC Buffer

Smooth muscle-specific expression of SV40 large TAg induces SMC proliferation causing adaptive arterial remodeling
Sindermann JR, et al.
American Journal of Physiology. Heart and Circulatory Physiology, 283(6), H2714-H2724 (2002)
Sambrook, J., et al.
Molecular Devices, 9-9 (1989)
Silicon nanonets for biological sensing applications with enhanced optical detection ability
Serre P, et al.
Biosensors And Bioelectronics, 68, 336-342 (2015)
Jürgen R Sindermann et al.
American journal of physiology. Heart and circulatory physiology, 283(6), H2714-H2724 (2002-10-22)
To study the effects of enhanced smooth muscle cell (SMC) proliferation on arterial vessel geometry in the absence of vessel trauma, we developed a transgenic mouse model expressing SV40 large T antigen under control of the 2.3-kb smooth muscle-myosin heavy
Joel W Slaton et al.
Molecular cancer research : MCR, 2(12), 712-721 (2005-01-07)
Protein serine/threonine kinase CK2 (formerly casein kinase 2) is a ubiquitous protein kinase that plays key roles in cell growth, proliferation, and survival. We have shown previously that its molecular down-regulation induces apoptosis in cancer cells in culture. Here, we
Exibir todos os documentos relacionados

Protocolos

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica