P1415

预杂交溶液

for Northern and Southern blotting, liquid (2x concentrate)

• NACRES: NA.25
• UNSPSC Code: 12161700

属性

• Quality Segment: 100, 200
• form: liquid (2x concentrate)
• foreign activity: DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
• storage temp.: -10 to -25°C

说明

General description

Sigma的预杂交液是一种2倍浓缩的即用型杂交缓冲液，适用于Northern和Southern印迹方案。

Application

适合用作Northern和Southern印迹方案的预杂交缓冲液。

Preparation Note

Sigma的2倍预杂交液含200 μg/mL DNA，溶剂为10倍SSC和2倍Denhardt溶液：

• 200 μg/mL DNA
• 2mg牛血清白蛋白(BSA)
• 2mg Ficoll
• 2mg聚乙烯吡咯烷酮(PVP)
• 0.15 M柠檬酸钠
• 1.5M NaCl

安全信息

• 存储类别: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable