I4657

Insulin-like Growth Factor-I Receptor human

≥95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

• Synonym(s): IGF-I Receptor
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD01323726

Properties

• biological source: human
• Quality Segment: 200
• recombinant: expressed in NSO cells
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• mol wt: α-chain ~120 & 150 kDa, β subunit ~46 & 50 kDa
• packaging: pkg of 50 μg
• impurities: endotoxin, tested
• UniProt accession no.: P08069
• storage temp.: −20°C
• Gene Information: human ... IGF1R(3480)

Description

General description

IGF1R (insulin-like growth factor I) is a receptor tyrosine kinase.[1] The gene is mapped to human chromosome 15q26.[2] It is expressed in various solid tumors as well as hematologic malignancies.[3]

Application

Insulin-like growth factor-I receptor human has been used to study the binding constants of estradiol to insulin, insulin receptors and insulin-like peptides.[4]

Biochem/physiol Actions

Binds IGF-I with high affinity, IGF-II with lower affinity, and insulin with weak affinity.

IGF-I receptor, a tyrosine kinase receptor with signal transduction pathways, is comprised of two α and two β subunits. IGF-I receptor binds IGF-I with high affinity, IGF-II with lower affinity, and insulin with weak affinity.

Physical form

Lyophilized from a 0.2 μm solution in phosphate buffered saline.

Analysis Note

As a result of glycosylation in SDS-PAGE under reducing conditions, the recombinant α subunit migrates as a doublet of ~120 kDa and 150 kDa and the β subunit migrates as doublet of ~46 kDa and 50 kDa.

The biological activity is measured by its ability to bind human IGF­-­I in a functional ELISA assay.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)