I2526

Insulin-like Growth Factor-II human

IGF-II, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): IGF-II
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00241895
• Biological source: human
• Recombinant: expressed in E. coli
• Assay: ≥95% (SDS-PAGE)
• Form: lyophilized powder
• Mol wt: monomer 7.5 kDa ((67 aa))
• Impurities: ≤0.1 EU/μg (LAL test)

Properties

• biological source: human
• Quality Segment: 200
• recombinant: expressed in E. coli
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤6 ng/mL ED₅₀
• quality: endotoxin tested
• mol wt: monomer 7.5 kDa ((67 aa))
• packaging: pkg of 5X50 μg, pkg of 50 μg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤0.1 EU/μg (LAL test)
• UniProt accession no.: P01344
• storage temp.: −20°C

Description

Biochem/physiol Actions

IGF-II, also known as multiplication stimulating activity (MSA), shows virtually identical bioactivities as IGF-I. IGF-II binds to the IGF-II receptor, IGF-I receptor, and weakly to the insulin receptor (IR). All IGF-II bioactivities in adult tissues are generally attributed to its interaction with IGF-I receptor. However, during embryogenesis the roles of both IGF-II receptor and IR may be significant in promoting normal growth.

Insulin-like growth factor II (IGF-II) also known as multiplication stimulating activity (MSA), shows virtually identical bioactivities as IGF-I. Expression of IGF-II is highest in fetal tissues, where it is believed to play a major role in overall growth and development. After birth IGF-II expression decreases to much lower levels in a variety of tissues. IGF-II is produced also in several tumors. IGF-II binds to IGF-II receptor (IGF-IIR), to IGF-IR and weakly to the insulin receptor (IR). All IGF-II bioactivities in adult tissues are generally attributed to its interaction with IGF-IR. However, during embryogenesis the roles of both IGF-IIR and IR may be significant in promoting normal growth.

Physical form

Lyophilized from a 0.2 µm filtered solution containing 0.1% Trifluoroacetic Acid (TFA).

Preparation Note

Reconstitute with sterile water at 0.1 mg/mL

Analysis Note

The biological activity is measured by a cell proliferation assay using Human breast cancer cell line MCF-7

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)