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5-Carboxyfluorescein N-succinimidyl ester

Name: 5-Carboxyfluorescein <I>N</I>-succinimidyl ester
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

Empirical Formula (Hill Notation):

C₂₅H₁₅NO₉

CAS Number:

92557-80-7

Molecular Weight:

473.39

UNSPSC Code:

12352108

NACRES:

NA.32

PubChem Substance ID:

57653496

MDL number:

MFCD00077322

Beilstein/REAXYS Number:

7865828

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Properties

product line

BioReagent

Quality Segment

100

assay

≥90% (HPLC)

form

powder

fluorescence

λ_ex 492 nm; λ_em 520 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

OC(=O)c1cc(ccc1C2=C3C=CC(=O)C=C3Oc4cc(O)ccc24)C(=O)ON5C(=O)CCC5=O

InChI

1S/C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)15-4-1-12(9-18(15)24(31)32)25(33)35-26-21(29)7-8-22(26)30/h1-6,9-11,27H,7-8H2,(H,31,32)

InChI key

TWOKNYRPKCLMAR-UHFFFAOYSA-N

Description

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Derivative of fluorescein giving stable derivative upon conjugation with biopolymers. Fluorescent probe for nucleotides[1][2]; Reagent for fluorescent derivatization in capillary electrophoresis

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1 of 1

This Item	21878	86826	88596
Sigma-Aldrich 92846 5-Carboxyfluorescein N-succinimidyl ester	Sigma-Aldrich 21878 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester	Sigma-Aldrich 86826 5-Carboxyfluorescein	Sigma-Aldrich 88596 Fluorescein-O′-acetic acid
suitability suitable for fluorescence	suitability suitable for fluorescence	suitability suitable for fluorescence	suitability suitable for fluorescence
fluorescence λ_ex 492 nm; λ_em 520 nm in 0.1 M Tris pH 8.0	fluorescence λ_ex 492 nm; λ_em 517 nm in DMF	fluorescence λ_ex 492 nm; λ_em 517 nm in 0.1 M Tris pH 8.0	fluorescence λ_ex 455 nm; λ_em 514 nm in 0.1 M Tris pH 8.0
form powder	form powder	form powder	form powder
Quality Level 100	Quality Level 200	Quality Level 100	Quality Level 100
storage temp. −20°C	storage temp. −20°C	storage temp. -	storage temp. -
assay ≥90% (HPLC)	assay ≥80% (HPLC)	assay ≥95% (HPLC)	assay ≥90% (HPCE)

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Lau, S.K., et al.

Journal of Chromatography A, 809, 203-203 (1998)

Nonradioactive labeling of RNA.

G L Igloi

Analytical biochemistry, 233(1), 124-129 (1996-01-01)

A modified ribodinucleotide (named pSEEp) has been synthesized using commercially available components on a DNA synthesizer. The presence of a 3'-terminal primary amino group permits its coupling to a range of nonradioactive labels, exemplified here by fluorescein. The product of

DNA sequencing with dye-labeled terminators and T7 DNA polymerase: effect of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragments.

L G Lee et al.

Nucleic acids research, 20(10), 2471-2483 (1992-05-25)

The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP alpha S's). The one-tube extension protocol was tested on single-stranded templates, as well as PCR fragments

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Global Trade Item Number

SKU	GTIN
92846-5MG-F	04061833270486

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