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5(6)-Carboxyfluorescein N-hydroxysuccinimide ester

Name: 5(6)-Carboxyfluorescein <I>N</I>-hydroxysuccinimide ester
Brand: SIGMA

BioReagent, suitable for fluorescence, mixture of isomers, ≥80% (HPLC)

Synonym(s):

5(6)-FAM SE, FLUOS

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About This Item

Empirical Formula (Hill Notation):

C₂₅H₁₅NO₉

CAS Number:

117548-22-8

Molecular Weight:

473.39

NACRES:

NA.32

PubChem Substance ID:

57647947

UNSPSC Code:

12352108

MDL number:

MFCD04119713

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Properties

Quality Segment

200

product line

BioReagent

assay

≥80% (HPLC)

form

powder

solubility

DMF: soluble, DMSO: soluble

fluorescence

λ_ex 492 nm; λ_em 517 nm in DMF

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

OC(=O)c1ccc(cc1C2=C3C=CC(=O)C=C3Oc4cc(O)ccc24)C(=O)ON5C(=O)CCC5=O.OC(=O)c6cccc(C(=O)ON7C(=O)CCC7=O)c6C8=C9C=CC(=O)C=C9Oc%10cc(O)ccc8%10

InChI

1S/2C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)18-9-12(1-4-15(18)24(31)32)25(33)35-26-21(29)7-8-22(26)30;27-12-4-6-14-18(10-12)34-19-11-13(28)5-7-15(19)22(14)23-16(24(31)32)2-1-3-17(23)25(33)35-26-20(29)8-9-21(26)30/h1-6,9-11,27H,7-8H2,(H,31,32);1-7,10-11,27H,8-9H2,(H,31,32)

InChI key

YVTPVJDECOUTJW-UHFFFAOYSA-N

Description

Application

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5(6)-FAM SE, FLUOS) may be used to prepare fluorescent derivatives of proteins such as tubulin and for the preparation of a phenytoin tracer for the determination of this and other drugs in serum and plasma by fluorescence polarization immunoassay (FPIA) and laser-induced fluorescence detection.

Fluorescent label for primary and secondary amine groups on proteins and other biomolecules.

Has been used to prepare fluorescent derivatives of tubulin and for the preparation of a phenytoin tracer for the determination of this drug in serum and plasma by fluorescence polarization immunoassay (FPIA)

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This Item	87444	46939	46935
Sigma-Aldrich 21878 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester	Sigma-Aldrich 87444 5-Carboxy-fluorescein diacetate N-succinimidyl ester	Sigma-Aldrich 46939 Fluorescein diacetate 5-maleimide	Sigma-Aldrich 46935 6-[Fluorescein-5(6)-carboxamido]hexanoic acid
suitability suitable for fluorescence	suitability suitable for fluorescence	suitability -	suitability suitable for fluorescence
fluorescence λ_ex 492 nm; λ_em 517 nm in DMF	fluorescence λ_ex 492 nm; λ_em 517 nm	fluorescence λ_ex 492 nm; λ_em 520 nm in 0.1 M phosphate pH 7.0 (after derivatization with 2-mercaptoethanol and after cleavage by esterase)	fluorescence λ_ex 491 nm; λ_em 515 nm in 0.1 M Tris pH 9.0
solubility DMF: soluble, DMSO: soluble	solubility DMF: soluble, DMSO: soluble	solubility DMF: soluble, DMSO: soluble, dioxane: soluble	solubility DMF: soluble, DMSO: soluble
form powder	form powder	form solid	form solid
Quality Level 200	Quality Level 100	Quality Level 100	Quality Level 200
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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