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07-681

Anti-phospho-AMPK α (Thr172) Antibody

Upstate®, from rabbit

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human, rat
Application:
WB
Citations:
32
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biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, rat

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (pThr172)

Gene Information

human ... PRKAA1(5562)

General description

Mr 63 kDa

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids surrounding phosphorylated Thr172 of human AMP-activated protein kinase alpha 2 (AMPKα2)

Application

Anti-phospho-AMPK α (Thr172) Antibody is an antibody against phospho-AMPK α (Thr172) for use in WB.

Biochem/physiol Actions

Recognizes phospho-AMPKα.

Physical form

Format: Purified

Analysis Note

Control
Serum starved HEK293 cells
routinely evaluated by immunoblot on RIPA lysates from overnight starved HEK293 cells and partially purified rat liver AMPK

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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This Item
06-99607-62607-363
antibody form

purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

human, rat

species reactivity

human, mouse, rat

species reactivity

human

species reactivity

human, rat

Gene Information

human ... PRKAA1(5562)

Gene Information

human ... CDKN1B(1027)

Gene Information

human ... PRKAA1(5562)
mouse ... Prkaa1(105787)

Gene Information

human ... PRKAA2(5563)

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: suitable


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Storage Class

10 - Combustible liquids

wgk

WGK 1



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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B E Kemp et al.
Biochemical Society transactions, 31(Pt 1), 162-168 (2003-01-28)
The AMP-activated protein kinase (AMPK) is a metabolic-stress-sensing protein kinase that regulates metabolism in response to energy demand and supply by directly phosphorylating rate-limiting enzymes in metabolic pathways as well as controlling gene expression.
J F P Wojtaszewski et al.
Biochemical Society transactions, 31(Pt 6), 1290-1294 (2003-12-04)
The AMPK (5'AMP-activated protein kinase) is becoming recognized as a critical regulator of energy metabolism. However, many of these effects in muscle metabolism have been ascribed to AMPK based on the use of the unspecific activator AICAR (5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside). Using mouse
Roles of 5'-AMP-activated protein kinase (AMPK) in mammalian glucose homoeostasis.
Rutter, Guy A, et al.
The Biochemical Journal, 375, 1-16 (2003)



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