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U1875

Sigma-Aldrich

Urease from Canavalia ensiformis (Jack bean)

Type III, glycerol solution, 500-800 units/mL

Szinonimák:

Jack bean urease, Urea amidohydrolase

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About This Item

CAS-szám:
9002-13-5
Enzyme Commission szám:
3.5.1.5 (BRENDA, IUBMB)
MDL-szám:
MFCD00070858
UNSPSC kód:
12352204
eCl@ss:
32160410
NACRES:
NA.54

biológiai forrás

Canavalia ensiformis

Minőségi szint

200

típus

Type III

form

glycerol solution

molekulatömeg

~544620

koncentráció

500-800 units/mL

tárolási hőmérséklet

2-8°C

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Általános leírás

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Urease of jack bean (Canavalia ensiformis), a hexamer of a single chain of 840 amino acid residues, is found in plants, bacteria, fungi, algae, and soil. It is considered the first nickel-containing enzyme and the first enzyme to be crystallized. Ureases exist in several isoforms, some are jack bean urease (JBURE-I), canatoxin (CNTX), and JBURE-IIB. JBURE-I is more abundant than the other two isoforms. Ureases are found in mature seeds.

Alkalmazás

Urease from Canavalia ensiformis (Jack bean) has been used:
  • to assay creatine and creatine phosphate using the sample extracted from the left ventricle (LV) and septal tissue
  • to determine the concentration of urea in a stabilized urine sample
  • as one of the standards to measure the urease activity of plant extracts

Biokémiai/fiziológiai hatások

In plants, ureases are essential for the bioavailability/metabolism of nitrogen in nature and defense mechanisms. Ureases are metalloenzymes that catalyze the hydrolysis of urea to release ammonia a nitrogen source for growth and carbon dioxide. They exhibit internal peptides-mediated insecticidal action and fungicidal activity.
Urease activity has been shown to be inhibited by benzpyrene following exposure to light. This inhibition was due to the effect of benzpyrene on urease′s sulfhydryl groups.

Egység definíció

One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)

Piktogramok

Health hazardExclamation mark
GHS08,GHS07

Figyelmeztetés

Danger

Figyelmeztető mondatok

H315,H319,H334,H335

Veszélyességi osztályok

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Célzott szervek

Respiratory system

Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Dokumentumtár megtekintése

Fernando Medina Ferrer et al.
Geobiology, 20(1), 79-97 (2021-08-03)
Modern carbonate tufa towers in the alkaline (~pH 9.5) Big Soda Lake (BSL), Nevada, exhibit rapid precipitation rates (exceeding 3 cm/year) and host diverse microbial communities. Geochemical indicators reveal that carbonate precipitation is, in part, promoted by the mixing of calcium-rich
Effect of light-activated benzpyrene on urease activity.
G C MILLS et al.
Cancer research, 13(1), 69-72 (1953-01-01)
Fernando Medina Ferrer et al.
Microbial biotechnology, 13(6), 1877-1888 (2020-07-29)
Microbial precipitation of calcium carbonate is a widespread environmental phenomenon that has diverse engineering applications, from building and soil restoration to carbon sequestration. Urease-mediated ureolysis and CO2 (de)hydration by carbonic anhydrase (CA) are known for their potential to precipitate carbonate
Melissa Postal et al.
Peptides, 38(1), 22-32 (2012-08-28)
Ureases (EC 3.5.1.5) are metalloenzymes that hydrolyze urea into ammonia and CO(2). These proteins have insecticidal and fungicidal effects not related to their enzymatic activity. The insecticidal activity of urease is mostly dependent on the release of internal peptides after
Pınar Tekiner et al.
Journal of molecular recognition : JMR, 25(11), 549-554 (2012-10-31)
Jack bean (Canavalia ensiformis) is the source of interesting proteins that contribute to modern biochemistry, and urease is the primary of these proteins. Owing to its role and occurrence in nature, urease has become a part of extensive studies. In
View All Related Papers

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Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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