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T9179
Terrific Broth
EZMix powder microbial growth medium
Szinonimák:
TB media
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
UNSPSC kód:
41106200
NACRES:
NA.85
Javasolt termékek
grade
for molecular biology
Minőségi szint
leírás
TB; quick dissolve, dust-free formulation
sterilitás
non-sterile
Forma
powder
technika/technikák
microbiological culture: suitable
alkalmazás(ok)
agriculture
food and beverages
microbiology
tárolási hőmérséklet
room temp
alkalmasság
nonselective for Escherichia coli
nonselective for coliforms
Általános leírás
Terrific Broth is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains glycerol, peptides, amino acids, water-soluble vitamins, and carbohydrates.
Alkalmazás
Suitable for non-selective cultivation of E. coli strains for cloning, high yield DNA plasmid production and high yield production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
Terrific broth has been used:
Terrific broth has been used:
- as a medium for selective growth of E.coli BL21 (DE3) V2RpRARE expressing MLL SET (nuclear proto-oncogene) domain in pET28GST-LIC
- to grow BL21 (DE3) cells containing plasmid expressing IL-8 (interleukin-8)
- Terrific broth is also suitable for use in bacterial suspension (Bacillus subtilis strain 1085B)
Tulajdonságok és előnyök
Terrific Broth EZMix powder provides:
- Granulated, dust-free format for safer handling and faster mixing
- A budget-friendly alternative to liquid
- Modified formulation (without glycerol)
- Easy scale-up using larger package sizes
Komponensek
*Note some of the product sizes available are given in liquid measure. This is a powder format product and those sizes reflect the final reconstituted volume.
23.6g/L Yeast Extract
11.8g/L Tryptone
9.4g/L K2HPO4
2.2g/L KH2PO4
23.6g/L Yeast Extract
11.8g/L Tryptone
9.4g/L K2HPO4
2.2g/L KH2PO4
Feloldás
Suspend 24.1 g of powder and 4 mL of glycerol in sufficient deionized water to make 500 mL. Autoclave for 15 minutes at 121 °C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Jogi információk
kapcsolódó termék
Product No.
Leírás
Árazás
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
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Analitikai tanúsítványok (COA)
Lot/Batch Number
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