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Key Documents

CMC0017

Sigma-Aldrich

OverExpress™ C41(DE3) Chemically Competent Cells

Escherichia coli, rod shaped

Szinonimák:

C41 strain

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(6)

About This Item

UNSPSC kód:
41106202
NACRES:
NA.85

product name

OverExpress™ C41(DE3) Chemically Competent Cells, for the highest protein expression

biológiai forrás

Escherichia coli

grade

for molecular biology

növekedési mód

adherent or suspension

morfológia

rod shaped

technika/technikák

microbiological culture: suitable

sejttranszformáció

competent cell type: chemically competent
transformation efficiency: ≥1 x 106 cfu/μg

kiszállítva

dry ice

tárolási hőmérséklet

−70°C

Related Categories

Általános leírás

OverExpress™ Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. The OverExpress™ strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). OverExpress C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Alkalmazás

OverExpress™ C41 (DE3) chemically competent cells have been used for expression screening.

Tulajdonságok és előnyök

  • Express genes cloned into any T7 vector with these BL21(DE3) derivatives
  • Effective in expressing toxic & membrane proteins
  • Cited in over 350 research articles

Komponensek

  • OverExpress C41(DE3) chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression


Jogi információk

OverExpress is a trademark of Imaxio, S.A.

kapcsolódó termék

Product No.
Leírás
Árazás

Tárolási osztály kódja

10 - Combustible liquids

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Rosetta 2(DE3) Competent Cells - Novagen Novagen′s Rosetta 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Sigma-Aldrich

71397

Rosetta 2(DE3) Competent Cells - Novagen

A high-throughput expression screening platform to optimize the production of antimicrobial peptides.
Schreiber C, et al.
Microbial cell factories, 16(1), 29-29 (2017)
Lily Goodyer Sait et al.
eLife, 9 (2020-10-23)
Voltage-gated sodium channels are targets for a range of pharmaceutical drugs developed for the treatment of neurological diseases. Cannabidiol (CBD), the non-psychoactive compound isolated from cannabis plants, was recently approved for treatment of two types of epilepsy associated with sodium
Ji-Young Youn et al.
Molecular biology of the cell, 21(17), 3054-3069 (2010-07-09)
BAR domains are protein modules that bind to membranes and promote membrane curvature. One type of BAR domain, the N-BAR domain, contains an additional N-terminal amphipathic helix, which contributes to membrane-binding and bending activities. The only known N-BAR-domain proteins in
Myles B C Dillon et al.
ACS chemical biology, 7(7), 1198-1204 (2012-04-18)
Protein arginine methyltransferases (PRMTs) catalyze the posttranslational methylation of arginine using S-adenosylmethionine (SAM) as a methyl-donor. The PRMT family is widely expressed and has been implicated in biological functions such as RNA splicing, transcriptional control, signal transduction, and DNA repair.
Altin Sula et al.
Molecular cell, 81(6), 1160-1169 (2021-01-28)
Voltage-gated sodium channels are targets for many analgesic and antiepileptic drugs whose therapeutic mechanisms and binding sites have been well characterized. We describe the identification of a previously unidentified receptor site within the NavMs voltage-gated sodium channel. Tamoxifen, an estrogen
View All Related Papers

Protocols

OverExpress™ Chemically Competent Cells

Protocol for OverExpress™ Chemically Competent Cells. Product Numbers: CMC0017, CMC0018, CMC0019, CMC0020, CMC0023, CMC0024

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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