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P1411

Sigma-Aldrich

Polymyxin B-Agarose

aqueous glycerol suspension

Szinonimák:

PMB

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez

Méret kiválasztása

5 ML
61 200,00 Ft
10 ML
118 000,00 Ft

61 200,00 Ft

Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.
Összes fotó(1)

Méret kiválasztása

Nézet módosítása
5 ML
61 200,00 Ft
10 ML
118 000,00 Ft

About This Item

MDL-szám:
MFCD00165491
UNSPSC kód:
41106500
NACRES:
NA.56

61 200,00 Ft

Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.

biológiai forrás

synthetic (organic)

Minőségi szint

200

Forma

aqueous glycerol suspension

a címkézés mértéke

~1 mg per mL

technika/technikák

affinity chromatography: suitable

Mátrix

Cross-linked 4% beaded agarose

mátrix aktiválás

cyanogen bromide

mátrix csatolmány

amino

mátrix távtartó

1 atom

kapacitás

200-500 μg/mL binding capacity (lipopolysaccharide)(from Escherichia coli serotype 0128:B12)

alkalmasság

suitable for chromatography

tárolási hőmérséklet

2-8°C

Alkalmazás

Polymyxin B-agarose is used in protein chromatography, affinity chromatography and specialty resins. Polymyxin B-agarose has been used to study periodontitis and endodontic infections as well as obesity and type 2 diabetes.
Useful for removal of endotoxins from solutions

Fizikai forma

Suspension in 50% glycerol containing 0.02% sodium azide

Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
0000375992
0000364940
0000348645
0000375992
0000364940

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Dokumentumtár megtekintése

L F Weikert et al.
American journal of physiology. Lung cellular and molecular physiology, 279(2), L216-L223 (2000-08-05)
Surfactant-associated protein A (SP-A) is involved in surfactant homeostasis and host defense in the lung. We have previously demonstrated that SP-A specifically binds to and enhances the ingestion of bacillus Calmette-Guerin (BCG) organisms by macrophages. In the current study, we
K Stuhlmeier et al.
Clinical and experimental immunology, 84(2), 317-323 (1991-05-01)
Coumarin as well as its derivatives 7-OH coumarin and 4-OH coumarin were found to stimulate interleukin-1 beta (IL-1 beta) release from freshly isolated human mononuclear cells (MNC) if the culture medium contained fetal calf serum. Under serum-free conditions, almost no
Samira B R Prado et al.
Scientific reports, 10(1), 1690-1690 (2020-02-06)
Dietary fibers have been shown to exert immune effects via interaction with pattern recognition receptors (PRR) such as toll-like receptors (TLR) and nucleotide-binding oligomerization domain (NOD)-like receptors. Pectin is a dietary fiber that interacts with PRR depending on its chemical
Sesselja Omarsdottir et al.
International immunopharmacology, 6(11), 1642-1650 (2006-09-19)
Many naturally occurring polysaccharides from fungi and lichens have been found to have immunomodulating activity. However, the majority of these studies have focused on their effects on the innate arm of the immune system. Although dendritic cells (DCs) belong to
C Moreno et al.
Clinical and experimental immunology, 76(2), 240-245 (1989-05-01)
We show here that purified lipoarabinomannan (LAM) from Mycobacterium tuberculosis can cause the release of tumour necrosis factor (TNF) in vitro from human blood monocytes and activated mouse peritoneal macrophages, and the production of TNF in vivo in mice pretreated
View All Related Papers

Questions

1–2 of 2 Questions  

  1. What polypropylene column should the we use according to the protocol to wash the resin prior to use?

    1 answer

    1. For washing the resin prior to use, the customer can use the following options:

      1. C2603 Mini-Columns: These have a capacity of 0.5 mL and an overall height of 1.9 inches.

      2. C2728 Mini-Columns: These have a capacity of 2.4 mL and an overall height of 1.5 inches.

      The washing procedure using Polymyxin B Agarose (Product No. P1411) can be carried out in a column or in a batch method. For a column, the customer can use disposable mini columns, such as Product Nos. C2603 (0.5 ml) and C2728 (5.0 ml). If a larger column is needed, the customer can use a polypropylene syringe barrel or use the batch method.

      In the batch method, the solution is incubated with the polymyxin B agarose for a minimum of 30 minutes with rotation in either a 15 ml or 50 ml polypropylene tube. The tube is then centrifuged, and the supernatant contains the protein of interest with decreased endotoxin that has been bound to the beads and pelleted in the tube.

      Helpful?

  2. What methods can be used to remove endotoxin from a product?

    1 answer

    1. The product is not tested for endotoxin; however, P1411 may be used to strip endotoxin. The P1411 datasheet includes a suggested protocol for this process. The protocol involves washing the resin, equilibrating the column, dissolving the sample in buffer, eluting with endotoxin-free buffer, and collecting the fractions. The collected fractions containing the protein should then be lyophilized. The polymyxin B agarose can be regenerated at least 10 times, and the resin can be stored in 25% ethanol at 4°C

      Helpful?

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