N4014

Nystatin

powder, suitable for cell culture, BioXtra

• Szinonimák: Fungicidin, Mycostatin
• CAS-szám: 1400-61-9
• EC-szám: 215-749-0
• MDL-szám: MFCD25541651
• UNSPSC kód: 12352207
• PubChem Substance ID: 24897362
• NACRES: NA.76

Tulajdonságok

• product name: Nystatin, powder, γ-irradiated, BioXtra, suitable for cell culture
• Minőségi szint: 200; 300
• sterilitás: γ-irradiated
• termékcsalád: BioXtra
• form: powder
• molekulatömeg: 926.1 g/mol
• technika/technikák: cell culture | mammalian: suitable
• antibiotikus hatásspektrum: fungi; yeast
• Hatásmechanizmus: cell membrane | interferes
• tárolási hőmérséklet: −20°C
• SMILES string: O[C@@H]([C@H](C)[C@H](C)O1)[C@@H](C)/C=C/C=C/CC/C=C/C=C/C=C/C=C/[C@H](O[C@@H]2O[C@H](C)[C@@H](O)[C@H](N)[C@@H]2O)C[C@@]3([H])[C@H](C(O)=O)[C@@H](O)C[C@](O)(O3)C[C@@H](O)[C@H](O)CC[C@@H](O)C[C@@H](O)C[C@@H](O)CC1=O
• InChI: 1S/C47H75NO17/c1-27-17-15-13-11-9-7-5-6-8-10-12-14-16-18-34(64-46-44(58)41(48)43(57)30(4)63-46)24-38-40(45(59)60)37(54)26-47(61,65-38)25-36(53)35(52)20-19-31(49)21-32(50)22-33(51)23-39(55)62-29(3)28(2)42(27)56/h5-6,8,10-18,27-38,40-44,46,49-54,56-58,61H,7,9,19-26,48H2,1-4H3,(H,59,60)/b6-5+,10-8+,13-11+,14-12+,17-15+,18-16+/t27-,28+,29-,30+,31+,32+,33+,34-,35+,36+,37-,38-,40+,41-,42+,43+,44-,46-,47+/m0/s1
• Nemzetközi kémiai azonosító kulcs: VQOXZBDYSJBXMA-QEKUPDCNSA-N

Leírás

Általános leírás

Chemical structure: polyene

Alkalmazás

Nystatin is a polyene antibiotic. Its uses include preventing cell culture contamination and inducing interleukin (IL)-1, IL-8, and tumor necrosis factor a secretion in TLR2-expressing THP1 cells. This product is recommended for use in cell culture applications at 50 mg/L.

Biokémiai/fiziológiai hatások

Mode of Action: Nystatin acts by binding to ergosterols in fungal cell membranes, increasing permeability of the membrane, and creating nystatin/ergosterol based ion channels. It also functions as a lipid raft-inhibiting reagent and as membrane associated cholesterol.

Antimicrobial spectrum: Nystatin acts against fungi, yeasts and molds.

Vigyázat

Nystatin in tissue culuture media is stable at 37°C for three days. It is also stable in moderately alkaline media. Solutions and aqueous suspensions begin to lose activity soon after preparation, with heat, light, and oxygen accelerating the decomposition. Aqueous suspensions are stable for 10 minutes when heating to 100°C at pH 7.

Elkészítési megjegyzés

Nystatin is soluble at 28 °C in methanol (11.2 mg/mL), ethanol (1.2 mg/mL), carbon tetrachloride (1.23 mg/mL), chloroform (0.48 mg/mL), benzene (0.28 mg/mL), and ethylene glycol (8.75 mg/mL). It is also soluble in DMSO at 5 mg/mL and freely soluble in DMF and formamide. It is not recommended to autoclave or sterile filter solutions of Nystatin. Nystatin is also effective as a suspension, stable at 37 °C for 3 days. Prepare 50 mg/mL stock suspensions in water and store at -20 °C. This product is γ-irradiated from N6261.

Egyéb megjegyzések

Keep container tightly closed in a dry and well-ventilated place.Store under inert gas.Air and light sensitive.Keep in a dry place

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 2
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)