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N3520

Sigma-Aldrich

Nutrient Mixture F-12 Ham

Kaighn′s Modification, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Szinonimák:

Ham’s F-12

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
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About This Item

MDL-szám:
MFCD00217417
UNSPSC kód:
12352207
NACRES:
NA.75

Forma

powder

technika/technikák

cell culture | mammalian: suitable

alkatrészek

NaHCO3: no
sodium pyruvate: 0.22 g/L
HEPES: no
L-glutamine: yes
phenol red: yes

kiszállítva

ambient

tárolási hőmérséklet

2-8°C

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Általános leírás

The nutrient mixture Ham′s F-12 is developed by Ham and it has higher levels of amino acids, vitamins and trace elements. Putrescine and linoleic acid are added to the formulation. Nutrient medium was originally designed for the serum-free growth of Chinese hamster ovary and lung cells. When used with whole or dialyzed serum or in combination with hormones and transferrin, it is widely used to grow mammalian and hybridoma cells.

Alkalmazás

Nutrient Mixture F-12 Ham is used for the following applications:
  • HeLa (human cervix adenocarcinoma cells) were grown in this medium (nutrient mixture F-12 Ham) supplemented with 10% heat-inactivated fetal calf serum
  • Used in cell culture for different cells during maintenance and incubation (i.e. A549 cell line was maintained as a monolayer in nutrient mixture F-12 HAM Medium along with other components)
  • Used in media for the propagation of Cowdria ruminantium
  • Used during preparation of primary mixed glial cultures

Kaighn′s modification of Ham′s F-12 and Coon′s F-12 has increased concentrations of amino acids and pyruvate, as well as modified salts (Konigsbergs). This medium was designed to support the growth of differentiated rat and chicken cells, and primary human liver cells.

Mennyiség

Formulated to contain 11.1 grams of powder per liter of medium.

Feloldás

Supplement with 2.5 g/L sodium bicarbonate.

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kiegészítés

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Piktogramok

Exclamation mark
GHS07

Figyelmeztetés

Warning

Figyelmeztető mondatok

H317

Veszélyességi osztályok

Skin Sens. 1

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
0000357587
0000357588
0000357440
0000357440
0000357588

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