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Merck

M4655

Sigma-Aldrich

Minimum Essential Medium Eagle

With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Szinonimák:

EMEM, MEM

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
12352207
NACRES:
NA.75

Minőségi szint

sterilitás

sterile-filtered

Forma

liquid

technika/technikák

cell culture | mammalian: suitable

szennyeződések

endotoxin, tested

alkatrészek

sodium pyruvate: no
phenol red: yes
Earle’s salts (5% CO2): yes
HEPES: no
NaHCO3: yes
L-glutamine: yes

kiszállítva

ambient

tárolási hőmérséklet

2-8°C

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Általános leírás

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.

Alkalmazás

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.

Egyéb megjegyzések

This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser.

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


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Analitikai tanúsítványok (COA)

Lot/Batch Number

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Dokumentumtár megtekintése

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Nucleic acids research, 48(12), 6740-6758 (2020-05-29)
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PLoS neglected tropical diseases, 12(4), e0006398-e0006398 (2018-04-17)
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Frontiers in immunology, 10, 3077-3077 (2020-02-11)
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Hepatocellular carcinoma (HCC) is a deadly form of liver cancer with high mortality worldwide. Unfortunately, the large heterogeneity of this disease makes it difficult to develop effective treatment strategies. Cellular network analyses have been employed to study heterogeneity in cancer

Cikkek

A kalcium egy ionilag stabil kétértékű kation, amely a sejtkultúrában fontos hasznos és toxikus tulajdonságokkal rendelkezik. A sejttenyésztési közegek széles skálájának összetevője.

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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