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M4892

SAFC

McCoy′s 5A Medium

Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Gyógyszeripari előállítás

Szinonimák:

5A basal medium

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About This Item

MDL-szám:
MFCD00217560
UNSPSC kód:
12352207
NACRES:
NA.71

Minőségi szint

500

form

powder

technika/technikák

cell culture | mammalian: suitable

alkatrészek

phenol red: 0.011 g/L
L-glutamine: 0.21915 g/L
glucose: 3.0 g/L (Dextro)
NaHCO3: no

kiszállítva

ambient

tárolási hőmérséklet

2-8°C

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Általános leírás

McCoy′s 5A Medium is recommended to produce viruses in primary cell cultures.

Alkalmazás

McCoy′s 5A Medium has been used for culturing:
  • human colon carcinoma cell lines (HCT116, RKO, FET, CBS, HCT116b, and TENN) to study the function of leucine-rich repeat-containing G-protein-coupled receptor (LGR5) in colon cancer metastasis
  • human colorectal HCT116 cells to study miR-224 over-expression in cancer
  • osteoblasts to study ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media

Biokémiai/fiziológiai hatások

McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

Mennyiség

Formulated to contain 11.9 grams of powder per liter of medium.

Feloldás

Supplement with 2.2 g/L sodium bicarbonate.

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kiegészítés

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Árazás

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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RPMI-1640 Medium With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

R8758

RPMI-1640 Medium

Sigma-Aldrich

Sigma-Aldrich

M0268

Minimum Essential Medium Eagle

PLTMax Human Platelet Lysate PLTMax Human Platelet Lysate is a growth factor rich supplement that is a superior alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) culture. 500ml.

Sigma-Aldrich

SCM142

PLTMax Human Platelet Lysate

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Cikkek

McCoy's 5A Modified Media Formulation

McCoy's 5A Modified Media Formulation

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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