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M0446

Sigma-Aldrich

Minimum Essential Medium Eagle, with Earl's salts

StableCell™, with Earle′s salts, stable glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Szinonimák:

Minimum Essential Medium Eagle, MEM

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About This Item

UNSPSC kód:
12352207
NACRES:
NA.75

product name

StableCell™ MEM, With Earle′s salts, stable glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Minőségi szint

400

sterilitás

sterile-filtered

form

liquid

technika/technikák

cell culture | mammalian: suitable

szennyeződések

endotoxin, tested

alkatrészek

sodium pyruvate: no
HEPES: no
Hanks’ salts (2% CO2): no
NaHCO3: yes
Earle’s salts (5% CO2): no
stable glutamine: yes
phenol red: yes
L-glutamine: no
glucose: yes

kiszállítva

ambient

tárolási hőmérséklet

2-8°C

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Általános leírás

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Alkalmazás

Minimum Essential Medium Eagle has been used in murine fibroblast culture for in vitro cytocompatibility testing.

Egyéb megjegyzések

This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser and L-Gln. It is supplemented with L-Ala-L-Gln dipeptide. This provides a more stable form of glutamine for cell culture. Free amino acid L-glutamine is known to be unstable in cell culture.

Jogi információk

StableCell is a trademark of Sigma-Aldrich Co. LLC

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javasolt

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Árazás

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Dokumentumtár megtekintése

Peter Minárik et al.
Materials science & engineering. C, Materials for biological applications, 73, 736-742 (2017-02-12)
Effect of processing by equal channel angular pressing (ECAP) on the degradation behaviour of extruded LAE442 magnesium alloy was investigated in a 0.1M NaCl solution, Kirkland's biocorrosion medium (KBM) and Minimum Essential Medium (MEM), both with and without 10% of
Jaroslav Čapek et al.
Materials science & engineering. C, Materials for biological applications, 79, 550-562 (2017-06-21)
Recently, iron-based materials have been considered as candidates for the fabrication of biodegradable load-bearing implants. Alloying with palladium has been found to be a suitable approach to enhance the insufficient corrosion rate of iron-based alloys. In this work, we have
A novel high-strength and highly corrosive biodegradable Fe-Pd alloy: Structural, mechanical and in vitro corrosion and cytotoxicity study
Capek J, et al.
Materials Science and Engineering, C, 79, 550-562 (2017)
Eva Pruchova et al.
Bioelectrochemistry (Amsterdam, Netherlands), 127, 26-34 (2019-01-18)
Titanium biomaterials are widely used in the medical field due to their biocompatibility and excellent corrosion and mechanical resistance. However, these materials have no antibacterial properties. To obtain an antibacterial active surface, a nanostructure of Ti6Al4V alloy was created. This
Monika Majerská et al.
Molecular pharmaceutics, 16(7), 3275-3283 (2019-05-24)
The lack of specificity of traditional cytostatics and increasing resistance of cancer cells represent important challenges in cancer therapy. One of the characteristics of cancer cells is their intrinsic oxidative stress caused by higher metabolic activity, mitochondrial malfunction, and oncogene
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