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Merck

D0819

Sigma-Aldrich

DMEM - high glucose

StableCell, with stable glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

Szinonimák:

Dulbecco’s Modified Eagle’s Medium - high glucose, DME, DMEM

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About This Item

UNSPSC kód:
12352207
NACRES:
NA.75

Terméknév

StableCell DMEM - high glucose, With 4500 mg/L glucose, stable glutamine, and sodium bicarbonate, without sodium pyruvate., liquid, sterile-filtered, suitable for cell culture

Minőségi szint

sterilitás

sterile-filtered

Forma

liquid

technika/technikák

cell culture | mammalian: suitable

alkalmasság

suitable for

alkatrészek

stable glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: no
glucose: high
NaHCO3: yes

kiszállítva

ambient

tárolási hőmérséklet

2-8°C

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Általános leírás

This DMEM-Hi glucose medium differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.

Alkalmazás

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Jogi információk

StableCell is a trademark of Sigma-Aldrich Co. LLC

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


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