Összes fotó(1)
Fontos dokumentumok
LYSISO1
Lysosome Isolation Kit
sufficient for 25 g (tissue), sufficient for 20 mL (packed cells), enrichment of lysosomes from tissues and packed cells
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
Javasolt termékek
Minőségi szint
használat
sufficient for 20 mL (packed cells)
sufficient for 25 g (tissue)
technika/technikák
centrifugation: suitable
fractionation: suitable
tárolási hőmérséklet
2-8°C
Általános leírás
Lysosomes are organelles ubiquitous in most prokaryotic and eukaryotic cells. They contain many acid hydrolases that take part in protein degradation in the cell. Lysosomes also contain lipases, nucleases and polysaccharidases and deficiencies in some of these enzymes lead to specific lysosomal storage diseases such as Tay Sachs, Gaucher, Hunter disease and others. They also contribute to maintaining cellular homeostasis. Malfunctions in this organelle directly impact cell behavior and fate. Lysosomes may also be involved in other cellular processes such as Albinism and aging.
Alkalmazás
Lysosome Isolation Kit has been used:
The Lysosome Isolation Kit provides a method for isolating lysosomes from animal tissues and from cultured cells by differential centrifugation followed by density gradient centrifugation and/or calcium precipitation. The presence of lysosomes can be measured by assaying the activity of the lysosomal marker Acid Phosphatase, with the Acid Phosphatase Assay Kit (Cat. No. CS0740). Separation from other organelles can be measured using the appropriate marker detection kits available from Sigma.
- in the isolation of cytosol and lysosome
- to obtain enriched light and heavy lysosomal fractions from cultured cells
- in the isolation of lysosomes of mouse bone marrow macrophages (BMMs)
- in lysosome purification
The Lysosome Isolation Kit provides a method for isolating lysosomes from animal tissues and from cultured cells by differential centrifugation followed by density gradient centrifugation and/or calcium precipitation. The presence of lysosomes can be measured by assaying the activity of the lysosomal marker Acid Phosphatase, with the Acid Phosphatase Assay Kit (Cat. No. CS0740). Separation from other organelles can be measured using the appropriate marker detection kits available from Sigma.
Tulajdonságok és előnyök
- Generates functional organelles for metabolism and disease research
- Includes protease inhibitor to manage degradation
- Measures integrity with included Neutral Red dye
- Choose from multiple options to obtain enriched fractions
- Enriched fractions for intact and functional lysosomes from tissues and cells
- Suitable for functional studies including protein degradation
Kit Components Also Available Separately
Product No.
Leírás
Biztonsági adatlap
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mLBiztonsági adatlap
Alkalmazás
Product No.
Leírás
Árazás
Figyelmeztetés
Warning
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Eye Irrit. 2 - Skin Irrit. 2
Tárolási osztály kódja
10 - Combustible liquids
Lobbanási pont (F)
188.6 °F
Lobbanási pont (C)
87 °C
Válasszon a legfrissebb verziók közül:
Analitikai tanúsítványok (COA)
Lot/Batch Number
Nem találja a megfelelő verziót?
Ha egy adott verzióra van szüksége, a tétel- vagy cikkszám alapján rákereshet egy adott tanúsítványra.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Az ügyfelek ezeket is megtekintették
Fang-Shin Nian et al.
Molecular neurobiology, 56(9), 6095-6105 (2019-02-06)
Mutations in RAB18, a member of small G protein, cause Warburg micro syndrome (WARBM), whose clinical features include vision impairment, postnatal microcephaly, and lower limb spasticity. Previously, our Rab18-/- mice exhibited hind limb weakness and spasticity as well as signs
Xiaowei Ma et al.
ACS nano, 5(11), 8629-8639 (2011-10-07)
Development of nanotechnology calls for a comprehensive understanding of the impact of nanomaterials on biological systems. Autophagy is a lysosome-based degradative pathway which plays an essential role in maintaining cellular homeostasis. Previous studies have shown that nanoparticles from various sources
Activation of Nlrp3 inflammasomes enhances macrophage lipid-deposition and migration: implication of a novel role of inflammasome in atherogenesis
Li X, et al.
PLoS ONE, 9(1), e87552-e87552 (2014)
Glycine N-methyltransferase deficiency affects Niemann-Pick type C2 protein stability and regulates hepatic cholesterol homeostasis
Liao YJ, et al.
Molecular Medicine, 18(3), 412-422 (2012)
Quantitative proteomic profiling for clarification of the crucial roles of lysosomes in microbial infections
Xu B, et al.
Molecular Immunology, 87(1), 122-131 (2017)
Cikkek
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
Lépjen kapcsolatba a szaktanácsadással