Összes fotó(2)
Fontos dokumentumok
L7658
LB Broth (Lennox)
EZMix powder microbial growth medium
Szinonimák:
Lennox broth
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
UNSPSC kód:
41106200
NACRES:
NA.85
Javasolt termékek
grade
for molecular biology
Minőségi szint
leírás
quick-dissolve, dust-free formulation
sterilitás
non-sterile
Forma
powder
technika/technikák
microbiological culture: suitable
pH
6.8-7.2(2.5% solution)
alkalmazás(ok)
agriculture
food and beverages
microbiology
tárolási hőmérséklet
room temp
alkalmasság
nonselective for Escherichia coli
nonselective for coliforms
Általános leírás
Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation.
Alkalmazás
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.
Tulajdonságok és előnyök
Lennox LB EZMix powder provides:
- Granulated, dust-free format for safer handling and faster mixing
- Convenient small package to eliminate weighing
- Easy scale-up using larger package sizes
- Standard formulation
Komponensek
*Note some of the product sizes available are given in liquid measure. This is a powder format product and those sizes reflect the final reconstituted volume.
10g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl
0.6 g/L inert binder (EZMix formulation only)
10g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl
0.6 g/L inert binder (EZMix formulation only)
Elkészítési megjegyzés
1. Suspend 20.6 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121 °C.
To prepare Lennox L Broth: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1M magnesium sulfate after autoclaving.
2. Autoclave for 15 minutes at 121 °C.
To prepare Lennox L Broth: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1M magnesium sulfate after autoclaving.
Feloldás
Stir to suspend 20.6g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Jogi információk
Zeocin is a registered trademark of Cayla Sarl
kapcsolódó termék
Product No.
Leírás
Árazás
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
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General protocols for growth of competent cells in microbial medium.
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