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G0751

Sigma-Aldrich

β-Glucuronidase from Helix pomatia

Type H-1, partially purified powder, ≥300,000 units/g solid

Szinonimák:

β-D-Glucuronide glucuronosohydrolase

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(7)

About This Item

CAS-szám:
9001-45-0
Enzyme Commission szám:
3.2.1.31 (BRENDA, IUBMB)
EC-szám:
232-606-8
MDL-szám:
MFCD00130629
UNSPSC kód:
12352204
NACRES:
NA.54

típus

Type H-1

Minőségi szint

200

form

partially purified powder

specifikus aktivitás

≥300,000 units/g solid

secondary activity

≥10,000 units/g solid sulfatase

tárolási hőmérséklet

−20°C

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Általános leírás

β-Glucuronidase Type H-1 from Helix pomatia is partially purified powder containing enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Alkalmazás

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Biokémiai/fiziológiai hatások

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Minőség

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Egység definíció

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

szubsztrát

Product No.
Leírás
Árazás

Piktogramok

Health hazard
GHS08

Figyelmeztetés

Danger

Figyelmeztető mondatok

H317,H334

Óvintézkedésre vonatkozó mondatok

P261 - P280 - P342 + P311

Veszélyességi osztályok

Resp. Sens. 1 - Skin Sens. 1

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, type N95 (US)

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Dokumentumtár megtekintése

M Richelle et al.
European journal of clinical nutrition, 53(1), 22-26 (1999-02-27)
To evaluate the plasma kinetics in man of epicatechin from black chocolate. An intervention study with 8 volunteers. Each served as his own control. Theobromine was used as control marker of the chocolate intake. Metabolic Unit, Nestle Research Center, Vers-chez-les-Blanc
Myriam Richelle et al.
The Journal of nutrition, 132(9), 2587-2592 (2002-09-11)
We investigated whether the bioavailability of isoflavones could be enhanced by enzymatic hydrolysis of glycosides to aglycones before consumption of a nonfermented soy food. Two drinks were formulated with an enriched isoflavone extract from soy germ (Fujiflavone P10), one of
D Briskey et al.
European journal of nutrition, 58(5), 2087-2097 (2018-07-06)
Curcumin has been shown to deliver protective effects against numerous degenerative conditions associated with high levels of inflammation and oxidative stress. Owing to its poor bioavailability when delivered orally, it is difficult to deliver a high concentration therapeutic dose. LipiSperse®
Shu-Shu Zhong et al.
ACS omega, 6(12), 8163-8170 (2021-04-06)
Arylsulfatase and β-glucuronidase are two important enzymes in humans, which play an important role in the dynamic equilibrium of steroidal estrogens. This work probably for the first time reported that hydrogen peroxide (H2O2), hypochlorite, and peracetic acid (PAA) could effectively
Kilia Y Liu et al.
The Journal of nutrition, 150(2), 222-230 (2019-09-10)
Vitamin E α-, γ-, or δ-tocopherol (αT, γT, δT) and γ- or δ-tocotrienol (γTE, δTE) are metabolized to hydroxychromanols and carboxychromanols including 13'-carboxychromanol (13'-COOH), 11'-COOH, and carboxyethyl hydroxychroman (CEHC), some of which have unique bioactivities compared with the vitamers. However
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Cikkek

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Protocols

ß-Glucuronidase Enzymatic Assay

Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.

UHPLC/MS for Drug Detection in Urine

Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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