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G8132

Sigma-Aldrich

β-Glucuronidase from limpets (Patella vulgata)

Type L-II, lyophilized powder, 1,000,000-3,000,000 units/g solid

Szinonimák:

β-D-Glucuronide glucuronosohydrolase

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(9)

About This Item

CAS-szám:
9001-45-0
Enzyme Commission szám:
3.2.1.31 (BRENDA, IUBMB)
EC-szám:
232-606-8
MDL-szám:
MFCD00130629
UNSPSC kód:
12352204
NACRES:
NA.54

biológiai forrás

limpet (Patella vulgata)

Minőségi szint

300

típus

Type L-II

Forma

lyophilized powder

specifikus aktivitás

1,000,000-3,000,000 units/g solid

kiszállítva

wet ice

tárolási hőmérséklet

−20°C

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Alkalmazás

β-Glucuronidase Type L-II has been shown to be a superior enzyme for the hydrolysis of drug-glucuronides from urine.
New Technical Article Comparing Performance of Different Enzymes
Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
The enzyme from patella vulgata is reported to be more effective in hydrolyzing opioid-glucuronides than the Helix pomatia, bovine liver and E. coli enzymes

Biokémiai/fiziológiai hatások

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption. Morphine 3-β-D glucuronide was converted to morphine using ß-glucuronidase obtained from Patella vulgate.

Minőség

Contains sulfatase activity which is inhibited by 0.1 M phosphate.

Egység definíció

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

Piktogramok

Health hazard
GHS08

Figyelmeztetés

Danger

Figyelmeztető mondatok

H317,H334

Óvintézkedésre vonatkozó mondatok

P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313

Veszélyességi osztályok

Resp. Sens. 1 - Skin Sens. 1

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, type N95 (US)

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
0000397186
0000378080
0000360999
0000361001
0000308733

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COA keresése

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Dokumentumtár megtekintése

Robert West et al.
Pain physician, 13(1), 71-78 (2010-02-02)
Physicians determine patient compliance with their medications by use of urine drug testing. It is known that measurement of benzodiazepines is limited by immunoassay specificity and cutoff limits and therefore does not offer physicians an accurate picture of their patients'
Vectors with the gus reporter gene for identifying and quantitating promoter regions in <I>Saccharomyces cerevisiae</I>
S.V. Marathe &amp; J.E. McEwen
Gene, 154, 105-107 (1994)
J D McCarter et al.
Current opinion in structural biology, 4(6), 885-892 (1994-12-01)
The determination of a large number of three-dimensional structures of glycosidases, both free and in complex with ligands, has provided valuable new insights into glycosidase catalysis, especially when coupled with results from studies of specifically labelled glycosidases and kinetic analyses
S Feng et al.
Journal of analytical toxicology, 25(7), 589-593 (2001-10-16)
Buprenorphine, which is a powerful analgesic, a substitution drug for opioids widely used in Europe, and a promising new drug currently undergoing clinical trials in the treatment of opioid dependence in the U.S., is excreted in human urine mainly as
T A Jennison et al.
Journal of analytical toxicology, 17(4), 208-210 (1993-07-01)
Methods to confirm morphine in urine require hydrolysis to liberate morphine from its 3-beta-D glucuronide (M-3G) conjugate. Lengthy enzyme hydrolysis procedures prolong testing turnaround time whereas rapid enzyme methods may produce a low conversion of M-3G to morphine. The purpose
View All Related Papers

Cikkek

Analysis of Drugs of Abuse in Urine

Validate urine sample cleanup with HLB SPE for opioid analysis via LC-MS/MS using the Supel™ Swift HLB 96-well plate.

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Protocols

ß-Glucuronidase Enzymatic Assay

Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.

UHPLC/MS for Drug Detection in Urine

Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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