Ugrás a tartalomra
Merck

C7805

Sigma-Aldrich

Monoclonal Anti-Collagen, Type III antibody produced in mouse

clone FH-7A, ascites fluid

Szinonimák:

Anti-Collagen III antibody

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

MDL-szám:
UNSPSC kód:
12352203
NACRES:
NA.41
konjugátum:
unconjugated
application:
DB
ELISA (i)
IHC (f)
WB
klón:
FH-7A, monoclonal
faj reaktivitás:
human, rat
citations:
54
technika/technikák:
dot blot: suitable
immunohistochemistry (frozen sections): 1-4,000 using frozen sections of human skin
indirect ELISA: suitable
western blot: suitable using a denatured-reduced preparation

biológiai forrás

mouse

Minőségi szint

konjugátum

unconjugated

antitest forma

ascites fluid

antitest terméktípus

primary antibodies

klón

FH-7A, monoclonal

molekulatömeg

antigen 70 kDa

tartalmaz

15 mM sodium azide

faj reaktivitás

human, rat

technika/technikák

dot blot: suitable
immunohistochemistry (frozen sections): 1-4,000 using frozen sections of human skin
indirect ELISA: suitable
western blot: suitable using a denatured-reduced preparation

izotípus

IgG1

UniProt elérési szám

kiszállítva

dry ice

tárolási hőmérséklet

−20°C

célzott transzláció utáni módosítás

unmodified

Géninformáció

human ... COL3A1(1281)
rat ... Col3a1(84032)

Általános leírás

Collagen is a fibrous protein present in the extracellular framework of all vertebrates. Collagen type III is a 300 kD molecule that belongs to fibrillar collagens family found mainly in skin, blood vessels, liver, placenta, tongue and thymus. This collagen is directly or indirectly involved in several genetic diseases like Ehlers-Danlos type IV disease. Monoclonal anti-collagen type III antibody can be used to determine the interstitial collagen I and III content in total protein homogenates. It is also used to study the immunolocalization of collagen type III in normal and neoplastic tissue. Mouse monoclonal anti-collagen type III antibody reacts specifically with native and denatured collagen type III but not with collagen types I, II, IV, V, VI, and X.
Collagen is a fibrous protein present in the extracellular framework of all vertebrates. Collagen type III is a 300 kD molecule that belongs to fibrillar collagens family found mainly in skin, blood vessels, liver, placenta, tongue and thymus. This collagen is directly or indirectly involved in several genetic diseases like Ehlers-Danlos type IV disease. Mouse monoclonal anti-collagen type III antibody reacts specifically with native and denatured collagen type III but not with collagen types I, II, IV, V, VI, and X. This product has also shown reactivity for rat and human collagen type III.
Monoclonal Anti-Collagen Type III (mouse IgG1 isotype) is derived from the FH-7A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human collagen type III.

Egyediség

Monoclonal Anti-Collagen Type III reacts specifically with native and denatured collagen type III. It does not recognize collagen types I, II, IV, V, VI, and X.

Immunogén

human collagen type III

Alkalmazás

Monoclonal Anti-Collagen, Type III antibody produced in mouse has also been used in enzyme linked immunosorbent assay (ELISA) and immunohistochemistry.
Monoclonal anti-collagen type III antibody has been used in immunofluorescence and western blot.

Biokémiai/fiziológiai hatások

This collagen is directly or indirectly involved in several genetic diseases like Ehlers-Danlos type IV disease. Monoclonal anti-collagen type III antibody can be used to determine the interstitial collagen I and III content in total protein homogenates.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 2

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


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Analitikai tanúsítványok (COA)

Lot/Batch Number

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Dokumentumtár megtekintése

R Mayne
Clinical biochemistry, 21(2), 111-115 (1988-04-01)
Monoclonal antibodies have recently been developed against all of the major collagen types isolated from both human and other species. These antibodies have several potential advantages over polyclonal antibodies, and a brief survey will be made of the different antibodies
Fluorescently labeled collagen binding proteins allow specific visualization of collagen in tissues and live cell culture
Krahn KN, et al.
Analytical Biochemistry, 350(2), 177-185 (2006)
Corinne N Riggin et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 37(9), 2035-2042 (2019-05-02)
Overuse-induced tendinopathy is highly prevalent in the general population. Percutaneous fenestration, or dry needling, techniques have been increasing in popularity, but despite their current use, there are no controlled laboratory studies to provide fundamental support for this practice. The objective
Advanced glycation end products as a source of artifacts in immunoenzymatic methods
Kuzan A, et al.
Glycoconjugate Journal, 35(1), 95-103 (2018)
Ana I Gonçalves et al.
PloS one, 8(12), e83734-e83734 (2014-01-05)
Current treatments for tendon injuries often fail to fully restore joint biomechanics leading to the recurrence of symptoms, and thus resulting in a significant health problem with a relevant social impact worldwide. Cell-based approaches involving the use of stem cells

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