Összes fotó(9)
Fontos dokumentumok
C5896
Choline Oxidase from Alcaligenes sp.
lyophilized powder, ≥10 units/mg solid
Szinonimák:
Choline: oxygen 1-oxidoreductase
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(9)
About This Item
Javasolt termékek
form
lyophilized powder
Minőségi szint
specifikus aktivitás
≥10 units/mg solid
molekulatömeg
~95 kDa
technika/technikák
cell based assay: suitable
alkalmasság
suitable for assay of choline
suitable for molecular biology
tárolási hőmérséklet
−20°C
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Alkalmazás
Choline oxidase from Alcaligenes sp. has been used:
- in a study to investigate a choline biosensor constructed with a chitinous membrane and its application in measuring cholinesterase inhibitory activities
- has also been used in spectroscopic studies on the photoreaction of choline oxidase with covalently bound flavin.
- as a component of phosphate buffer saline (PBS) buffer for measurement of choline acetyltransferase (ChAT) activity in brain tissue homogenates
- to measure the choline generated by autotaxin (ATX) by ATX activity assay and phospholipase-D (PLD) by PLD activity assay
Biokémiai/fiziológiai hatások
Choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as the intermediate and molecular oxygen as th eprimary electron acceptor. The enzyme can also accept betaine-aldehyde as a substrate. This allows the study of the reaction mechanism for the conversion of choline to the aldehyde intermediate, & of betaine-aldehyde to glycine-betaine. The enzyme is a flavoprotein with a molecular weight of approx. 72,000 Da (gel filtration) or 66,000 Da (SDS gel electrophoresis).
Fizikai tulajdonságok
Isoelectric point:4.1 ± 0.1
Michaelis constants:2.84 x 10¯3M (Choline), 5.33 x 10¯3M (Betaine aldehyde)
Structure:One mol of FAD is covalently bound to mol of the enzyme
Inhibitors:p-Chloromercuribenzoate, Cu++, Co++, Hg++, Ag+
Optimum pH:8.0 – 8.5
Optimum temp:40 – 45°C
pH Stability:pH 7.0 – 9.0 (30°C, 2hr)
Thermal stability:Below 37°C (pH 7.5, 10min)
Michaelis constants:2.84 x 10¯3M (Choline), 5.33 x 10¯3M (Betaine aldehyde)
Structure:One mol of FAD is covalently bound to mol of the enzyme
Inhibitors:p-Chloromercuribenzoate, Cu++, Co++, Hg++, Ag+
Optimum pH:8.0 – 8.5
Optimum temp:40 – 45°C
pH Stability:pH 7.0 – 9.0 (30°C, 2hr)
Thermal stability:Below 37°C (pH 7.5, 10min)
Egység definíció
One unit will form 1 μmole of H2O2 with oxidation of 1 μmole of choline to betaine aldehyde per min at pH 8.0 at 37 °C. Note: During the conversion of choline to betaine by choline oxidase, 2 μmoles of H2O2 are produced for every μmole of choline.
kapcsolódó termék
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Resp. Sens. 1
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Egyéni védőeszköz
Eyeshields, Gloves, type N95 (US)
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az ügyfelek ezeket is megtekintették
M Ohta-Fukuyama et al.
Journal of biochemistry, 88(1), 197-203 (1980-07-01)
Choline oxidase from Alcaligenes sp. catalyzed the oxidation of choline and betaine aldehyde to betaine with concomitant consumption of oxygen and production of hydrogen peroxide. The values of Km for choline and betaine aldehyde were 0.87 and 6.2 mM, respectively.
Rungaroon Waditee et al.
The Journal of biological chemistry, 278(7), 4932-4942 (2002-12-06)
Glycine betaine (N,N,N-trimethylglycine) is an important osmoprotectant and is synthesized in response to abiotic stresses. Although almost all known biosynthetic pathways of betaine are two-step oxidation of choline, here we isolated two N-methyltransferase genes from a halotolerant cyanobacterium Aphanothece halophytica.
Samantha R Eck et al.
Biological psychiatry, 88(7), 566-575 (2020-07-01)
Stress exacerbates symptoms of schizophrenia and attention-deficit/hyperactivity disorder, which are characterized by impairments in sustained attention. Yet how stress regulates attention remains largely unexplored. We investigated whether a 6-day variable stressor altered sustained attention and the cholinergic attention system in
M C Carou et al.
Acta histochemica, 119(5), 462-470 (2017-05-17)
Follicular atresia in granulosa and theca cells occurs by apoptosis through weak hormonal stimulation. We have previously proposed an in vitro model to study this process by inducing apoptosis in BGC-1, a bovine granulosa cell line, and in primary cultures
S Pundir et al.
Analytical biochemistry, 427(1), 26-32 (2012-05-09)
A bienzymatic choline biosensor was constructed by coimmobilizing acetylcholinesterase (AChE) and choline oxidase (ChO) onto nanocomposite of carboxylated multiwalled carbon nanotubes (c-MWCNTs) and zirconium oxide nanoparticles (ZrO(2)NPs) electrodeposited on the surface of a glassy carbon electrode (GCE) and using it
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