Összes fotó(8)
Fontos dokumentumok
G7141
Glucose Oxidase from Aspergillus niger
Type X-S, lyophilized powder, 100,000-250,000 units/g solid (without added oxygen)
Szinonimák:
β-D-Glucose:oxygen 1-oxidoreductase, G.Od., GOx
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(8)
About This Item
Javasolt termékek
típus
Type X-S
Minőségi szint
Forma
lyophilized powder
specifikus aktivitás
100,000-250,000 units/g solid (without added oxygen)
molekulatömeg
160 kDa
összetétel
Protein, ≥65%
környezetbarátabb alternatív termék tulajdonságai
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
alkalmazás(ok)
diagnostic assay manufacturing
idegen aktivitás
Catalase ≤5 units/mg protein
környezetbarátabb alternatív kategória
tárolási hőmérséklet
−20°C
InChI
1S/C6H12O6/c7-1-2-3(8)4(9)5(10)6(11)12-2/h2-11H,1H2/t2-,3-,4+,5-,6-/m1/s1
Nemzetközi kémiai azonosító kulcs
WQZGKKKJIJFFOK-VFUOTHLCSA-N
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Általános leírás
Mass of glucose oxidase (GOX), a flavoprotein ranges from approximately 130 to 175 kDa. Some fungi and insects are capable of producing GOX.
Molecular Weight: 160 kDa (gel filtration)
pI: 4.2
Extinction coefficient: E1% = 16.7 (280 nm)
Glucose oxidase from Aspergillus niger is a dimer consisting of 2 equal subunits with a molecular mass of 80 kDa each. Each subunit contains one flavin adenine dinulceotide moiety and one iron. The enzyme is a glycoprotein containing ~16% neutral sugar and 2% amino sugars. The enzyme also contains 3 cysteine residues and 8 potential sites for N-linked glycosylation.
Glucose oxidase is capable of oxidizing D-aldohexoses, monodeoxy-D-glucoses, and methyl-D-glucoses at varying rates.
The pH optimum for glucose oxidase is 5.5, while it has a broad activity range of pH 4-7. Glucose oxidase is specific for β-D-glucose with a KM of 33-110 mM.
Glucose oxidase does not require any activators, but it is inhibited by Ag+, Hg2+, Cu2+, phenylmercuric acetate, and p-chloromercuribenzoate. It is not inhibited by the nonmetallic SH reagents: N-ethylmaleimide, iodoacetate, and iodoacetamide.
Glucose oxidase can be utilized in the enzymatic determination of D-glucose in solution. As glucose oxidase oxidizes β-D-glucose to D-gluconolactate and hydrogen peroxide, horseradish peroxidase is often used as the coupling enzyme for glucose determination. Although glucose oxidase is specific for β-D-glucose, solutions of D-glucose can be quantified as α-D-glucose will mutorotate to β-D-glucose as the β-D-glucose is consumed by the enzymatic reaction.
pI: 4.2
Extinction coefficient: E1% = 16.7 (280 nm)
Glucose oxidase from Aspergillus niger is a dimer consisting of 2 equal subunits with a molecular mass of 80 kDa each. Each subunit contains one flavin adenine dinulceotide moiety and one iron. The enzyme is a glycoprotein containing ~16% neutral sugar and 2% amino sugars. The enzyme also contains 3 cysteine residues and 8 potential sites for N-linked glycosylation.
Glucose oxidase is capable of oxidizing D-aldohexoses, monodeoxy-D-glucoses, and methyl-D-glucoses at varying rates.
The pH optimum for glucose oxidase is 5.5, while it has a broad activity range of pH 4-7. Glucose oxidase is specific for β-D-glucose with a KM of 33-110 mM.
Glucose oxidase does not require any activators, but it is inhibited by Ag+, Hg2+, Cu2+, phenylmercuric acetate, and p-chloromercuribenzoate. It is not inhibited by the nonmetallic SH reagents: N-ethylmaleimide, iodoacetate, and iodoacetamide.
Glucose oxidase can be utilized in the enzymatic determination of D-glucose in solution. As glucose oxidase oxidizes β-D-glucose to D-gluconolactate and hydrogen peroxide, horseradish peroxidase is often used as the coupling enzyme for glucose determination. Although glucose oxidase is specific for β-D-glucose, solutions of D-glucose can be quantified as α-D-glucose will mutorotate to β-D-glucose as the β-D-glucose is consumed by the enzymatic reaction.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in fuel cell research. For more information see the article in biofiles.
Alkalmazás
Glucose Oxidase from Aspergillus niger has been used:
- in the (glucose oxidase) GO reagent to measure the glucose content by the glucose oxidase (GO) method
- to activate the human renal carcinoma cell line for constructing the oxidative stress model
- to study its influence in the paste on the analytical performance of the bioelectrode
Glucose oxidase is widely used in the food and pharmaceutical industries as well as a major component of glucose biosensors.
Biokémiai/fiziológiai hatások
Glucose oxidase (GOX) oxidizes D-glucose to D-gluconolactone and hydrogen peroxide. Hydrogen peroxide, the catalytic product of GOX, serves as an anti-bacterial and anti-fungal agent. It is safe and can be used in several industrial applications like baking, dry egg powder production, wine production, gluconic acid production, etc. It possesses electrochemical activity, which makes it extremely important in glucose sensors and in fuel cell applications.
Glucose oxidase catalyses the oxidation of β-d-glucose to d-glucono-β-lactone and hydrogen peroxide, with molecular oxygen as an electron acceptor.
Minőség
May contain traces of amylase, maltase, glycogenase, invertase, and galactose oxidase.
Egység definíció
One unit will oxidize 1.0 μmole of β-D-glucose to D-gluconolactone and H2O2 per min at pH 5.1 at 35 °C, equivalent to an O2 uptake of 22.4 μl per min. If the reaction mixture is saturated with oxygen, the activity may increase by up to 100%.
Analízis megjegyzés
Protein determined by biuret
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Resp. Sens. 1
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Egyéni védőeszköz
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Protocols
Glucose oxidase activity measured via continuous spectrophotometric assay at 500 nm, indicating glucose oxidation rate.
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