Összes fotó(7)
Fontos dokumentumok
C2888
Acetylcholinesterase from Electrophorus electricus (electric eel)
Type V-S, lyophilized powder, ≥1,000 units/mg protein
Szinonimák:
AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(7)
About This Item
Javasolt termékek
biológiai forrás
Electrophorus electricus
Minőségi szint
típus
Type V-S
Forma
lyophilized powder
specifikus aktivitás
≥1,000 units/mg protein
molekulatömeg
280 kDa
összetétel
Protein, ≥45% biuret
pI
~5.3
oldhatóság
Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)
tárolási hőmérséklet
−20°C
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Általános leírás
Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)
Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)
Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.
Alkalmazás
Acetylcholinesterase from Electrophorus electricus (electric eel) has been used:
- in acetylcholinesterase activity assay
- to study its effects on Xenopus embryonic hippocampal cells
- to study the effect of acetylcholine inhibitors on development and plasticity
Biokémiai/fiziológiai hatások
Acetylcholinesterase (AChE) exhibits its activity against acetylcholine in the neuromuscular junctions and synaptic cleft of cholinergic neurons. It participates in the nitric oxide signaling pathway.
Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.
Vigyázat
For dilute solutions (<1 mg/ml), add 1 mg/ml BSA to stabilize. Solutions containing at least 1 mg protein/ml in dilute neutral phosphate buffer are stable at least 6 months refrigerated.
Egység definíció
One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.
Fizikai forma
Lyophilized powder containing Tris buffer salts
Analízis megjegyzés
The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.
Egyéb megjegyzések
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Egyéni védőeszköz
Eyeshields, Gloves, type N95 (US)
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