A9210

Annexin V FITC Conjugate from human placenta

• Szinonimák: Annexin V, Calphosbindin I, Lipocortin V, PAP-1
• UNSPSC kód: 41106305
• NACRES: NA.32

Tulajdonságok

• form: buffered aqueous solution
• Minőségi szint: 300
• a címkézés mértéke: 1-2 mol FITC per mol annexin V
• UniProt elérési szám: P08758
• kiszállítva: wet ice
• tárolási hőmérséklet: 2-8°C
• Géninformáció: human ... ANXA5(308)

Leírás

Alkalmazás

For detection of apoptotic cells by flow cytometry or fluorescence microscopy.

Biokémiai/fiziológiai hatások

Annexins are ubiquitous homologous proteins that bind phospholipids in the presence of calcium. The cellular changes involved in the apoptotic process include loss of phospholipid asymmetry during the early stages. This phenomenon is universal and is not limited to stimulus or to mammalian cells, but also occurs in insect and plant cells. In living cells, phosphatidylserine is transported to the inside of the lipid bilayer by the Mg²⁺ATP dependent enzyme, aminophospholipid translocase. At the onset of apoptosis, phosphatidylserine becomes translocated to the external surface of the cell membrane. Since the movement of phosphatidylserine from the internal membrane surface to the external surface is an early indicator of apoptosis, annexin V and its conjugates that interact strongly and specifically with phosphatidylserine may be used to detect apoptosis. Annexin V conjugates can be used to detect apoptotic cells significantly earlier than DNA-based assays. Fluorescent dye labeled annexin V have applications in flow cytometry, fluorescence microscopy, and laser scanning cytometry.

Kiszerelés

Package size based on protein content

Fizikai forma

Solution in 50 mM Tris-HCl, pH 7.5, containing 100 mM NaCl.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable