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APOAC

Sigma-Aldrich

Annexin V-Cy3™ Apoptosis Detection Kit

Szinonimák:

Red fluorescent Annexin V apoptosis assay

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About This Item

UNSPSC kód:
12352207
NACRES:
NA.32

használat

sufficient for 200 tests

Minőségi szint

200

kiszerelés

pkg of 1 kit

technika/technikák

flow cytometry: suitable

alkalmazás(ok)

cell analysis
detection

észlelési módszer

fluorometric

kiszállítva

wet ice

tárolási hőmérséklet

2-8°C

Tulajdonságok és előnyök

Detects apoptosis earlier in the process than DNA-based assays such as TUNEL
  • Rapid labeling of cells. Cell staining takes only 10 minutes.
  • No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
  • 6-Carboxyfluorescein diacetate secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

Elv

Sigma′s Annexin V-Cy3 kit allows detection of annexin V bound to apoptotic cells by fluorescence microscopy. The Annexin V-Cy3 kit uses the dye Cy3.18 as the fluorochrome conjugated with annexin V. By microscopy, Cy3.18 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that the cells are viable. This combination allows the differentiation among early apoptotic cells (annexin V positive, 6-CFDA positive), necrotic cells (annexin V positive, 6-CFDA negative), and viable cells (annexin V negative, 6-CFDA positive).

Jogi információk

Cy3 is a trademark of Cytiva

Kit Components Only

Product No.
Leírás
  • Annexin V Cy3.18 Conjugate from human placenta, buffered aqueous solution 10 μg

Kit Components Also Available Separately

Product No.
Leírás
Biztonsági adatlap
  • C50416-Carboxyfluorescein diacetate 10 mgBiztonsági adatlap

Tárolási osztály kódja

10 - Combustible liquids

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Cikkek

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Apoptózis vizsgálatok

Celluláris apoptózis vizsgálatok a programozott sejthalál kimutatására Annexin V, kaszpáz és TUNEL DNS-fragmentációs vizsgálatok segítségével.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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