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APOAF

Sigma-Aldrich

Annexin V-FITC Apoptosis Detection Kit

Szinonimák:

Annexin V-FITC, Apoptosis probe FITC

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)

About This Item

UNSPSC kód:
12352207
NACRES:
NA.84

használat

(20 tests)

Minőségi szint

300

kiszerelés

pkg of 1 kit

technika/technikák

flow cytometry: suitable

alkalmazás(ok)

cell analysis
detection

észlelési módszer

fluorometric

kiszállítva

wet ice

tárolási hőmérséklet

2-8°C

Általános leírás

Annexin V-FITC kit allows fluorescent detection of annexin V bound to apoptotic cells and quantitative determination by flow cytometry. The AnnexinV-FITC kit uses annexin V conjugated with fluorescein isothiocyante (FITC) to label phosphatidylserine sites on the membrane surface. The kit includes propidium iodide (PI) to label the cellular DNA in necrotic cells where the cell membrane has been totally compromised. This combination allows the differentiation among early apoptotic cells (annexin V positive, PI negative), necrotic cells (annexin V positive, PI positive), and viable cells (annexin V negative, PI negative).

Alkalmazás

Annexin V-FITC Apoptosis Detection Kit was used:
  • in staining of LNCaP prostate cancer cells for measuring the G. lucidum extracts activity during the treatment of prostate cancer.
  • for tumor cell labelling to study the inhibitory activity of DBP-maf (Vitamin D binding protein-macrophage activating factor) on prostate tumor cells.
  • for indirect measurement of flippase activity.

Tulajdonságok és előnyök

Detects apoptosis earlier in the process than DNA-based assays such as TUNEL.
  • Rapid labeling of cells. Cell staining takes only 10 minutes.
  • No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
  • Propidium iodide secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

Egyéb megjegyzések

Allow all components to reach room temperature before use.

Kit Components Only

Product No.
Leírás
  • APOAFA

kapcsolódó termék

Product No.
Leírás
Árazás

08168

Timestrip Plus™ 8 °C

Tárolási osztály kódja

10 - Combustible liquids

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
0000389724
0000378388
0000378389
0000378388
0000378389

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COA keresése

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Anti-Annexin V antibody, Mouse monoclonal clone AN5, purified from hybridoma cell culture

Sigma-Aldrich

A8604

Anti-Annexin V antibody, Mouse monoclonal

Supelco

Supelco

04511

Live/Dead Cell Double Staining Kit

Denise C Arruda et al.
The Journal of biological chemistry, 287(18), 14912-14922 (2012-02-16)
Complementarity-determining regions (CDRs) from monoclonal antibodies tested as synthetic peptides display anti-infective and antitumor activities, independent of the specificity of the native antibody. Previously, we have shown that the synthetic peptide C7H2, based on the heavy chain CDR 2 from
Juliana Rizzo et al.
Eukaryotic cell, 13(6), 715-726 (2013-12-18)
Flippases are key regulators of membrane asymmetry and secretory mechanisms. Vesicular polysaccharide secretion is essential for the pathogenic mechanisms of Cryptococcus neoformans. On the basis of the observations that flippases are required for polysaccharide secretion in plants and the putative
Kalvin J Gregory et al.
PloS one, 5(10), e13428-e13428 (2010-10-27)
Vitamin D binding protein-macrophage activating factor (DBP-maf) is a potent inhibitor of tumor growth. Its activity, however, has been attributed to indirect mechanisms such as boosting the immune response by activating macrophages and inhibiting the blood vessel growth necessary for
Ben-Zion Zaidman et al.
International journal of oncology, 31(4), 959-967 (2007-09-06)
Ganoderma lucidum (Curt.:Fr.) P. Karst, a medicinal fungus, has been widely used in Asian countries for centuries to prevent or treat a variety of diseases, including cancer. However, the mechanisms responsible for the effects of G. lucidum on cancer cells
Jing-Fang Hong et al.
Molecular medicine reports, 13(6), 4541-4548 (2016-04-16)
The aim of the present study was to investigate the in vitro and in vivo anticancer and apoptotic effects of taraxerol acetate in U87 human glioblastoma cells. The effects on cell cycle phase distribution, cell cycle-associated proteins, autophagy, DNA fragmentation and cell migration were
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Cikkek

Quality Control Considerations in Cell Culture

Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Apoptózis vizsgálatok

Celluláris apoptózis vizsgálatok a programozott sejthalál kimutatására Annexin V, kaszpáz és TUNEL DNS-fragmentációs vizsgálatok segítségével.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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