A2064

Anti-Human IgG−Alkaline Phosphatase antibody, Mouse monoclonal

clone GG-5, purified from hybridoma cell culture

• Szinonimák: Monoclonal Anti-Human IgG (γ-chain specific)
• UNSPSC kód: 12352203
• NACRES: NA.46

Tulajdonságok

• biológiai forrás: mouse
• Minőségi szint: 200
• konjugátum: alkaline phosphatase conjugate
• antitest forma: purified immunoglobulin
• antitest terméktípus: secondary antibodies
• klón: GG-5, monoclonal
• Forma: buffered aqueous glycerol solution
• faj reaktivitás: human
• technika/technikák: direct ELISA: 1:50,000; dot blot: 1:80,000; immunohistochemistry: suitable; western blot (chemiluminescent): 1:80,000
• izotípus: IgG1
• kiszállítva: wet ice
• tárolási hőmérséklet: 2-8°C
• célzott transzláció utáni módosítás: unmodified

Leírás

Általános leírás

Binds human IgG; does not bind other human Igs.The antibody is specific for a determinant on the heavy chain of human IgG. This antibody will form antigen-antibody complexes in the liquid phase in the presence of 3% PEG 6000.

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.

Monoclonal Anti-Human IgG (Γ-chain specific) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Alkalmazás

Anti-Human IgG- Alkaline Phosphatase antibody, Mouse monoclonal has been used in:

• enzyme linked immunosorbent assay (ELISA)
• western blotting
• dot blot
• immunohistology

Elisa assays were performed using alkaline phosphatase conjugated monoclonal mouse anti-human IgG to detect antibodies against various S. Aureus antigens in the serum from healthy individuals. The antibody was diluted 1:30000 in PBSt and incubated with samples for 2 hours at 37 degrees. Reactin was developed using p-nitrophenyl phosphate substrate (Sigma).

Fizikai forma

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 2
• Egyéni védőeszköz: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)