I6758

IPTG

≥99% (TLC), ≤0.1% Dioxane

• Szinonimák: Isopropyl β-D-1-thiogalactopyranoside, IPTG, Isopropyl β-D-thiogalactoside
• Tapasztalati képlet (Hill-képlet): C₉H₁₈O₅S
• CAS-szám: 367-93-1
• Molekulatömeg: 238.30
• Beilstein: 4631
• EC-szám: 206-703-0
• MDL-szám: MFCD00063273
• UNSPSC kód: 12352201
• PubChem Substance ID: 24896093
• NACRES: NA.31

Tulajdonságok

• biológiai forrás: synthetic
• Minőségi szint: 300
• leírás: non-ionic
• Teszt: ≥99% (TLC)
• form: powder
• technika/technikák: thin layer chromatography (TLC): suitable
• szennyeződések: ≤0.1% Dioxane
• szín: white to off-white
• hasznos pH-tartomány: 5-7 (50 g/L)
• mp: 105 °C ((221 °F))
• oldhatóság: water: 0.05 g/L, clear
• alkalmazás(ok): cell analysis; life science and biopharma
• tárolási hőmérséklet: 2-8°C
• SMILES string: CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O
• InChI: 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
• Nemzetközi kémiai azonosító kulcs: BPHPUYQFMNQIOC-NXRLNHOXSA-N

Leírás

Általános leírás

Isopropyl β-D-Thiogalactopyranoside (IPTG) is a molecular biology reagent used to induce gene expression in bacterial systems. It mimics lactose, binding to the lac repressor protein, leading to gene activation. IPTG is crucial for studying gene regulation, protein expression, and recombinant protein production, providing valuable insights into cellular and molecular biology research.

Alkalmazás

IPTG has been used:

• in the induction of protein expression in Lactobacillus reuteri GroEL and mutants
• in the induction of protein expression in Escherichia coli

IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

Biokémiai/fiziológiai hatások

IPTG is a non-metabolizable analog of allolactose. It acts as an inducer that regulates the transcriptional activity of the lac promoter, a promoter that is used for the expression of the protein in E.coli.

Egyéb megjegyzések

Non-metabolizable galactose analog.

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Feloldás

A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM.

Biztonsági adatok

• Piktogramok: GHS08
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H350
• Óvintézkedésre vonatkozó mondatok: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Veszélyességi osztályok: Carc. 1B
• Tárolási osztály kódja: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)