LMDH-RO

L-Malate Dehydrogenase (L-MDH)

from pig heart (mitochondrial)

• Enzyme Commission szám: 1.1.1.37 (BRENDA, IUBMB)
• UNSPSC kód: 12352202

Tulajdonságok

• biológiai forrás: Porcine heart (mitochondrial)
• Minőségi szint: 100
• form: solution; suspension
• specifikus aktivitás: ~1200 units/mg protein (At 25 °C with oxaloacetate as the substrate.)
• kiszerelés: pkg of 1 mL (10127248001 solution); pkg of 1 mL (10127256001 suspension); pkg of 5 mL (10127914001suspension)
• gyártó/kereskedő neve: Roche
• technika/technikák: activity assay: suitable
• szín: white
• optimális pH: 7.4-7.5(reduction of oxaloacetate); 9.2-9.5(oxidation of malate)
• oldhatóság: water: miscible
• alkalmasság: suitable for Western blot
• NCBI elérési szám: NM_001244153.1
• UniProt elérési szám: P00346
• alkalmazás(ok): life science and biopharma
• idegen aktivitás: Fumarase <0.01%; GIDH 0.001%; GOT 0.001%; GPT 0.00185%; LDH <0.01%; NADH oxidase <0.001%
• kiszállítva: wet ice
• tárolási hőmérséklet: 2-8°C
• Géninformáció: Porcine ... MDH2(397039)

Leírás

Általános leírás

L-malate:NAD⁺ oxidoreductase
The L-malate dehydrogenase enzyme is a nuclear gene product that is synthesized with a 24-residue amino-terminal signal peptide. This peptide is proteolytically cleaved during the translocation of the enzyme to the mitochondrial matrix.

Alkalmazás

The enzyme L-malate dehydrogenase from pig heart has been used to measure PEPCK (phosphoenolpyruvate carboxykinase) activity. The oxaloacetate, produced by PEPCK, is reduced to malate via the oxidation of NADH, which in turn is measured at 340 nm using a spectrophotometer. The enzyme has also been used to measure oxaloacetate by measuring the reduction in NADH spectroscopically at 340 nm.

Biokémiai/fiziológiai hatások

The enzyme L-malate dehydrogenase from pig heart catalyzes the oxidation of L-malate to oxaloacetate. The enzyme is an NAD-dependent mitochondrial dehydrogenase that functions in the tricarboxylic acid cycle. It is a component of the malate-aspartate shuttle that transports reducing equivalents across the inner mitochondrial membrane in the form of malate.

Minőség

Contaminants: <0.002% GOT, <0.01% fumarase and LDH, each luM each, <0.02% HK, <0.002% PGI

Fizikai forma

Solution in 50% glycerol (v/v), pH approximately 7

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Elkészítési megjegyzés

Activator: – phosphate
– arsenate
– Mg²⁺
– Zn²⁺

Egyéb megjegyzések

For life science research only. Not for use in diagnostic procedures.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 1
• Lobbanási pont (F): No data available
• Lobbanási pont (C): No data available