10128023001

NADH

Grade II, disodium salt

• Szinonimák: β-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate, β-DPNH, β-NADH, DPNH, Diphosphopyridine nucleotide, reduced form, NADH
• Tapasztalati képlet (Hill-képlet): C₂₁H₂₇N₇Na₂O₁₄P₂ · xH₂O
• CAS-szám: 606-68-8
• Molekulatömeg: 709.40 (anhydrous basis)
• MDL-szám: MFCD03791273
• UNSPSC kód: 12352204
• PubChem Substance ID: 329748955

Tulajdonságok

• Minőségi szint: 100
• Teszt: 98%
• Forma: amorphous powder
• molekulatömeg: (NADH-Na₂: M_r = 709.4; NADH: M_r = 665.4)
• kiszerelés: pkg of 1 g
• gyártó/kereskedő neve: Roche
• tárolási körülmény: protect from light
• λmax: 260 and 340 nm
• kiszállítva: wet ice
• SMILES string: O.[Na+].[Na+].NC(=O)C1=CN(C=CC1)[C@@H]2O[C@H](COP([O-])(=O)OP([O-])(=O)OC[C@H]3O[C@H]([C@H](O)[C@@H]3O)n4cnc5c(N)ncnc45)[C@@H](O)[C@H]2O
• InChI: 1S/C21H29N7O14P2.2Na.H2O/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33;;;/h1,3-4,7-8,10-11,13-16,20-21,29-32H,2,5-6H2,(H2,23,33)(H,34,35)(H,36,37)(H2,22,24,25);;;1H2/q;2*+1;/p-2/t10-,11-,13-,14-,15-,16-,20-,21-;;;/m1.../s1
• Nemzetközi kémiai azonosító kulcs: FWBNCIFELNNJCX-UHOVGGJYSA-L

Leírás

Alkalmazás

As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.

NADH has been used in MDH (malate dehydrogenase) enzymatic activity assay.

NADH has been used in lactate dehydrogenase (LDH) enzymatic activity assay, glutamate-cysteine ligase activity assay and fructose 2,6-bisphosphate assay.

Biokémiai/fiziológiai hatások

NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, β-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD⁺/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease.
As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.

Kiszerelés

Packaged by solid weight.

Minőség

Purity: 98% β-NADH-Na₂ based on dry weight (enzymatically and absorbance at 340 nm), 99% (absorbance at 260 nm), A₂₆₀ nm-A₃₄₀ nm: <2.40
Organic solvents: <4% (GC)

Specifikációk

Absorbance coefficients: 14.3 x 10³ l x mol^-1 x cm^-1 at 260 nm, 6.18 x 10³ l x mol^-1 x cm^-1 at 334 nm (Hg), 6.3 x 10³ l x mol^-1 x cm^-1 at 340 nm, 3.4 x 10³ l x mol^-1 x cm^-1 at 365 nm (Hg)

Formula: C₂₁H₂₇N₇O₁₄P₂Na₂

pH value in water: 7.5 ± 0.5 (100 mg/ml)

Fizikai forma

Amorphous powder

Elkészítési megjegyzés

Activator: Mg²⁺ (5 to 10 mM)
Storage conditions (working solution): 10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADH after 4 weeks at 2 to 8 °C

Tárolás és stabilitás

Store at 2 to 8 °C. (Store dry!)

Egyéb megjegyzések

For life science research only. Not for use in diagnostic procedures.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable