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12041677001

Roche

CDP-Star®, ready-to-use

>98%, solution, suitable for dot blot, suitable for Northern blotting, suitable for Southern blotting

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)

About This Item

UNSPSC kód:
12352204

leírás

Disodium 2-chloro-5-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo[3.3.1.13,7 ]decan}-4-yl)-1-phenyl phosphate

Minőségi szint

100

Teszt

>98%

Forma

solution

kiszerelés

bottle of 2 × 50 mL ([0.25 mM])

gyártó/kereskedő neve

Roche

technika/technikák

Northern blotting: suitable
Southern blotting: suitable
dot blot: suitable

tárolási hőmérséklet

2-8°C

Közzététel

Purity: Purified by HPLC.

Alkalmazás

Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots:
  • Southern blot
  • Northern blot
  • Dot blot
  • Colony and plaque hybridization
  • Gel shift assays
Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots:
  • Southern blot
  • Northern blot
  • Dot blot
  • Colony and plaque hybridization
  • Gel shift assays
  • Chemiluminescent detection.

Tulajdonságok és előnyök

Contents
0.25 mM solution (0.124 mg/ml), ready-to-use, colorless solution
CDP-Star is a chemiluminescent substrate for alkaline phosphatase that enables fast, ultra-sensitive detection of biomolecules by producing extremely rapid visible light. Light emission is recorded on X-ray film or on luminescence imager systems.
  • Save time with a fast, ultra-sensitive substrate.
  • Easily strip and reprobe membranes.
  • Take multiple exposures of up to two days for each experiment.
Reuse the substrate by filtering and storing in sodium azide.

Elkészítési megjegyzés

Storage conditions (working solution): Washing Buffer: 15 to 25 °C, stable
Maleic Acid Buffer: 15 to 25 °C, stable
Detection Buffer: 15 to 25 °C, stable
Blocking Solution: always prepare freshly
Antibody Solution: 2 to 8 °C

Egyéb megjegyzések

  • First exposure to X-ray film should be 5-15 minutes. Examine film and then determine other exposure times based on initial result.
  • No preincubation step required.
  • Apply substrate quickly to avoid gray shadows from substrate drops and drying of membrane (leads to uneven, high background).
  • Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
  • Exposure times are shorter than with CSPD.
  • Do not use nitrocellulose membranes.
Repeat exposures can be made up to two days after the addition of substrate.
For life science research only. Not for use in diagnostic procedures.

Jogi információk

CDP-Star is a registered trademark of Tropix, Inc.

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

nwg

Lobbanási pont (F)

does not flash

Lobbanási pont (C)

does not flash

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Analitikai tanúsítványok (COA)

Lot/Batch Number

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Non-Radioactive Detection of Trinucleotide Repeat Size Variability.
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Detection of expanded RNA repeats using thermostable group II intron reverse transcriptase.
Carrell ST, et al.
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